Figure 6: Metformin increases Bcl-2 expression on HIV-infected and uninfected T-cells.
The HIV-1NL4.3Bal infection in vitro was performed as described in Figure 3 on cells from HIV-uninfected participants. Cells collected at day 12 post-infection were stained on the surface with CD4 antibodies and intracellularly with HIV-p24 and Bcl-2 antibodies and analyzed by flow cytometry. Shown are (A) levels of Bcl-2 expression on uninfected (CD4highHIV-p24−) and productively infected (CD4lowHIV-p24+) T-cells in one representative donor and (B) statistical analysis of Bcl-2 expression (GeoMFI) relative to the medium condition (considered 1). Each symbol represents one donor (n=4; median ± interquartile range). Friedman and uncorrected Dunn’s multiple comparison p-values are indicated on the graphs.