Figure 3: Fusion transcript detection applied to SeraCare v4 Fusion Reference Control sample.

(a) Quantities of PacBio long reads and TruSeq Illumina short reads identified as evidence for each of the 16 control fusions as ascertained by CTAT-LR-fusion and FusionInspector, respectively, across each sample replicate. PacBio replicate reads were downsampled to match the number of sequenced bases from the respective Illumina replicate samples. (b) Binary heatmap for the identification of the 16 control fusions pairs in different fusion detection software according to each of the three replicates of long read sequences, using all (not downsampled) sequenced reads. PacBio replicates are ordered (a) left to right or (b) top to bottom as MAS-ISO-seq monomer (replicate 1), and MAS-ISO-seq 8mer-concatamer sequenced replicates 2 and 3. Counts of sequenced reads are provided in Supplementary Table S1.