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Performance characteristics of SARS-CoV-2 RT-qPCR, HAdV 40/41 and Bacteroides HF183 qPCR assays.
| Assays | qPCR/RT-qPCR performance characteristics | |||
|---|---|---|---|---|
| Efficiency (E) (%) | Linearity (r2) | Slope | Y-intercept | |
| SARS-CoV-2 | 93.4 | 0.994 | −3.490 | 40.50 |
| HAdV 40/41 | 96.0 | 1.000 | −3.411 | 39.31 |
| Bacteroides HF183 | 90.1 | 0.999 | −3.586 | 42.97 |
Efficiency: −1 + 10(−1/slope).
