Figure 2.

CCG-203971 and CCG-232601 inhibit Rho/MRTF/SRF signaling. WI-38 cells were treated with 20 µM of CCG molecules for 24 h. (A) Representative Western blots show the protein expression of RhoA, MRTF-A, MRTF-B, p49/STRAP, and SRF; GAPDH was used as a loading control. (B) Quantification of relative protein levels normalized against GAPDH was shown in the graphs. Data are mean ± SD of three repeats. Significant difference * p  <  0.05; ** p  <  0.01; *** p  <  0.001; **** p  <  0.0001 by one-way ANOVA with Tukey’s procedure.