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. 2024 Feb 23;16(5):626. doi: 10.3390/nu16050626

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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NGS of PCLSs, cultured for up to 96 h in WEGG or GFIPO. (A) Volcano plots representing the differentially expressed genes (red), with a threshold of log2FoldChange > 1 on the x-axis and p.adj < 0.05 (−Log10p.adj > 1.30) on the y-axis, comparing GFIPO and WEGG at each time point. WEGG serves as the control group. (B,C) Venn diagrams illustrating the distribution of up-regulated genes (B) and down-regulated genes (C) across all time points. (D,E) Heatmap showing the number of overlapped up-regulated (D) and down-regulated (E) DEGs between two timepoints; overlap of the same timepoint stands for the unique DEGs amount as shown in the Venn diagrams. (F) The top 10 up-regulated genes (highlighted in pink, right) and down-regulated genes (highlighted in black, left) at each time point.