Extended Data Fig. 7. In vivo methylation profile of the top three DMRs identified in vitro from the ZFP-ETRs versus mock comparison.
Dot plots reporting the percentage of in vivo methylation at single-CpG resolution in ZFP-ETR- and vehicle-treated mice by targeted BS-seq. Two genomic sites were interrogated corresponding to the top two DMRs identified in vitro from the ZFP-ETRs versus mock comparison. DMR-3, -4 and -5 were identified in vitro as associated with the Pcsk9 promoter region. The in vivo methylation levels of these DMRs were quantified producing a single PCR including all the three DMRs. Each dot represents a single CpG in the indicated DMRs (mean of n = 3 for each experimental group).