Table 1.

Primer sequences for quantitative real-time PCR analysis.

Target gene(s)	Amplicon length (bp)	Primer sequence
NF-κB	78	Forward primer: 5′-CTGGTGGACACATACAGGAAGAC-3′
		Reverse primer: 5′-ATAGGCACTGTCTTCTTTCACCTC-3′
TNF-α	246	Forward primer: 5′-ACCCTCACACTCACAAACCA-3′
		Reverse primer: 5′-GGCAGAGAGGAGGTTGACTT-3′
IL-6	542	Forward primer: 5′-CTGGTGACAACCACGGCCTTCCCTA-3′
		Reverse primer: 5′-ATGCTTAGGCATAACGCACTAGGTT-3′
HO-1	127	Forward primer: 5′-TTCAGAAGGGCCAGGTGACC-3′
		Reverse primer: 5′-AAGTAGACAGGGGCGAAGACTGG-3′
Nrf2	225	Forward primer: 5′-ATGATGGACTTGGAGCTGCC -3′
		Reverse primer: 5′-TTGTAACTGAGCGAAAAAGGCTTT-3′
Mucin 1	133	Forward primer: 5′-ACCTACCATCCTATGAGCGAG-3′
		Reverse primer: 5′-GGTTTGTGTAAGAGAGGCTGC-3′
Mucin 2	70	Forward primer: 5′-TGAAGACCTGCGGCTGTGT-3′
		Reverse primer: 5′-CAGTCGAACTCGAAGTGCTCC-3′
Occludin	138	Forward primer: 5′-TCACTTTTCCTGCGGTGACT-3′
		Reverse primer: 5′-GGGAACGTGGCCGATATAATG-3′
Claudin-1	210	Forward primer: 5′-ATGCAAAGATGTTTTGCCAC-3′
		Reverse primer: 5′-TACAAATTCCCATTGCAGCCC-3′
Beta actin	118	Forward primer: 5′GGGAATGGGTCAGAAGGACT-3′
		Reverse primer: 5′-CTTCTCCATGTCGTCCCAGT-3′
A. muciniphila	329	Forward primer: 5′-CAGCACGTGAAGGTGGGGAC-3′
		Reverse primer: 5′-CCTTGCGGTTGGCTTCAGAT-3′
Universal bacteria	118	Forward primer: 5′GGGAATGGGTCAGAAGGACT-3′
		Reverse primer: 5′-CTTCTCCATGTCGTCCCAGT-3′