Physiology Correction for “Late-life shift in caloric intake affects fly metabolism and longevity,” by Michael Li, Jacob Macro, Kali Meadows, Dushyant Mishra, Dominique Martin, Sara Olson, Billy Joe Huggins, Brenton R. Graveley, James Y. H. Li, and Blanka Rogina, which published December 8, 2023; 10.1073/pnas.2311019120 (Proc. Natl. Acad. Sci. U.S.A. 120, (50) e2311019120).
The authors note that Fig. 3 A and C and SI Appendix, Fig. S4 A–C appeared incorrectly. The labels and units on the y axis for both figures were incorrect. The published units on the y axis “(mg/mg fly weight)” have been replaced with “(µg/mg fly weight)” (micrograms/milligram fly weight). The previously published data listed on the y axis are for 10 flies, and the previously published values should therefore be divided by 10 as shown here. The statistical analysis and conclusions are the same.
Fig. 3.
Age-dependent effects of shift on metabolism. (A) Levels of TAG in male flies on days 20 and 50 before shifting, and 1, 2, and 5 d after shifting from H to L diet (HL) or L to H diet (LH). (B) Tissue-specific DE gene analysis of count data of Agpat2, Dgat2, ACC, AcCoAS, Pepck2 (Phosphoenolpyruvate Carboxykinase 2), Hnf4 (Hepatocyte nuclear factor 4), SREBP, Lip3 (Lipase 3), and dob (doppelganger von brummer) during HL and LH shift. (C) Levels of glucose in male flies on days 20 and 50 before shifting, and 1, 2, and 5 d after shifting from H to L diet (HL) or L to H diet (LH). Error bars represent SEM. Data from 20 and 50 d were analyzed separately using two-way ANOVA. Post-hoc analysis was conducted using Tukey’s test, correcting for multiple comparisons. Results represent means ± SE of three biological replicates containing 10 flies per replicate. P: 0.033 (*), 0.002 (**), 0.0002 (***), <0.0001 (****).
Additionally, the authors note that the legends for Datasets S6–S10 appeared incorrectly. The Datasets have listed levels of triglycerides (S6), glucose (S7), glycogen (S8), trehalose (S9), and proteins (S10) for 10 flies.
The corrected Fig. 3 and its legend appear below. The online version has been corrected, and the SI Appendix has been corrected online.