Fig. 1.

OVX procedure and verification in mice. (A) Diagram of the procedure used for animal model construction. The mice were divided into 2 groups, and only the ovaries of those in the OVX group were removed. After eight weeks, the behavioural phenotype was observed, and specimens were harvested. (B) Oestradiol levels in the serum of arteriovenous mixed blood; n = 11 biologically independent animals per group. The data are shown as the means ± SEMs. Student's t-test was used to analyse the differences between the two groups; ***P < 0.001.