REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal anti-BrdU	Developmental Studies Hybridoma Bank	Cat#G3G4; RRID: AB_2314035
Rabbit monoclonal anti-Olfm4	Cell Signaling	Cat#D6Y5A; RRID: AB_2650511
Mouse monoclonal anti-β-catenin	BD Biosciences	Cat#610154; RRID: AB_397555
Mouse monoclonal anti-E-cadherin	BD Biosciences	Cat#610181; RRID: AB_397580
Goat polyclonal anti-ezrin	Tebu Bio	Cat#ER-14-0503; RRID: AB_1545407
Mouse monoclonal anti-transferrin receptor (H68.4)	Thermo Fisher Scientific	Cat#13–6800; RRID: AB_2533029
Rabbit polyclonal anti-phospho-ERM (Ezrin, Thr567; Radixin, Thr564; Moesin, Thr558)	Cell Signaling Technology	Cat#3141; RRID: AB_330232
Rabbit polyclonal anti-Cbr3 (Crumbs Cell Polarity Complex Component 3)	Dr. Benjamin L. Margolis Laboratory (University of Michigan Medical School, Ann Arbor, Michigan, USA)	N/A
Rabbit polyclonal anti-lysozyme	Dako	Cat#N1515
Mouse monoclonal anti-RhoA	Cytoskeleton	Cat#ARH03; RRID: AB_10708069
Mouse monoclonal anti-beta tubulin (TUB 2.1)	Sigma-Aldrich	Cat#T4026; RRID: AB_477577
Donkey anti-goat IgG (H + L) Alexa Fluor 594	Thermo Fisher Scientific	Cat#A-11058; RRID: AB_2534105
Goat anti-rabbit IgG (H + L) Alexa Fluor 594	Thermo Fisher Scientific	Cat# A-11012; RRID: AB_2534079
Goat anti-rabbit IgG (H + L) Alexa Fluor 488	Thermo Fisher Scientific	Cat# A-11008; RRID: AB_143165
Goat anti-mouse IgG (H + L) Alexa Fluor 488	Thermo Fisher Scientific	Cat# A-11001; RRID: AB_2534069
Chemicals, peptides, and recombinant proteins
DAPI (4′, 6-Diamidino-2-phenylindole dihydrochloride)	Biochemica	Cat#A1001
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine	Sigma-Aldrich	Cat#B8503
p-Nitro-Blue tetrazolium chloride	Sigma-Aldrich	Cat#N6876
Levamisole hydrochloride	Santa Cruz Biotechnology	Cat#sc-205730
1-methoxy-5-methylphenazinium methyl sulfate	Sigma-Aldrich	Cat#M8640
Alcian blue 8GX	Sigma-Aldrich	Cat#A5268
p-Nitrophenyl Phosphate Liquid Substrate System	Sigma-Aldrich	Cat#N7653
D-(+)-xylose	Sigma-Aldrich	Cat#X1500
FITC-dextran	Sigma-Aldrich	Cat#FD4
IWR-1-endo	STEMCELL Technologies	Cat#72564
EdU (5-ethynyl-2′-deoxyuridine)	Lumiprobe	Cat#10540
Sulfo-Cy3-Azide	Lumiprobe	Cat#A1330
7-AAD (7-Aminoactinomycin D)	FisherScientific	Cat#10748784
Critical commercial assays
NovoLink polymer detection system	Leica Biosystems	Cat#RE7280
Dako Fluorescence Mounting Medium	Agilent	Cat#S302380-2
Bouin’s solution	Sigma-Aldrich	Cat#HT10132
TRI Reagent	Thermo Fisher Scientific	Cat#AM9738
High Capacity cDNA Reverse Transcription Kit	Applied Biosystems	Cat#4368814
SYBR Select Master Mix	Applied Biosystems	Cat#4472919
TaqMan Universal Master Mix II	Applied Biosystems	Cat#4440047
RhoA Pull-Down Activation Assay Biochem Kit	Cytoskeleton	Cat#BK036
Experimental models: Cell lines
Vil-Cre+;RhoAT19N and Vil-Cre–;RhoAT19N intestinal organoids	This manuscript	N/A
Experimental models: Organisms/strains
Mouse: RhoAT19N: C57BL/6JJcl-CAT-RhoA-DN/4-18	RIKEN BioResource Research Center	RRID: IMSR_RBRC01295
Mouse: Vil-Cre+: C57BL/6J.SJL-Tg(Vil1-cre)997Gum/J	The Jackson Laboratory	RRID: IMSR_JAX:018963
Oligonucleotides
Table S1	This paper	N/A
Software and algorithms
ImageJ	ImageJ	RRID: SCR_003070
FCS Express 4.0	De Novo Software	RRID: SCR_016431
QuPath	QuPath	RRID: SCR_018257