Figure 3.

Sleep deprivation alters DVB-dependent spicule protraction behavior

(A) Cartoon of neurons and muscles controlling spicule protraction in male tail. DVB is shown in dark purple and SPC is shown in light purple.

(B) Cartoon depicting mechanism for aldicarb induced spicule protraction with opposing functions of DVB GABAergic inhibition (red) and SPC cholinergic excitation (green).

(C) Time to spicule protraction following aldicarb exposure in control, aptf-1, and aptf-1; nrx-1(del) day 1 males.

(D) Time to spicule protraction following aldicarb exposure in control, npr-1, and npr-1; nrx-1(del) day 1 males.

(E) Time to spicule protraction following aldicarb exposure in control day 1 males and control and nrx-1(del) day 1 males that underwent L4 vibration sleep deprivation. Number of individual animals is indicated by “n”, p values from one-way ANOVA with Tukey’s post-hoc test shown, error bars represent SEM.