Abstract
Introduction
Acetaldehyde dehydrogenase 2 (ALDH2) had reported as a prominent role in the development of cardiometabolic diseases among Asians. Our study aims to investigate the relationship between ALDH2 polymorphism and cardiometabolic risk factors in East Asian population.
Method
We searched databases of PubMed, Web of Science, and Embase updated to Oct 30th, 2023. We extracted data of BMI, Hypertension, SBP, DBP, T2DM, FBG, PPG, HbA1c, TG, TC, LDL-C and HDL-C.
Result
In total, 46 studies were finally included in our meta-analysis, containing, 54068 GG and, 36820 GA/AA participants. All outcomes related to blood pressure revealed significant results (hypertension OR=0.83 [0.80, 0.86]; SBP MD=-1.48 [-1.82, -1.14]; DBP MD=-1.09 [-1.58, -0.61]). FBG showed a significant difference (MD=-0.10 [-0.13, -0.07]), and the lipid resulted significantly in some outcomes (TG MD=-0.07 [-0.09, -0.04]; LDL-C MD=-0.04 [-0.05, -0.02]). As for subgroups analysis, we found that in populations without severe cardiac-cerebral vascular diseases (CCVDs), GG demonstrated a significantly higher incidence of T2DM (T2DM OR=0.88 [0.79, 0.97]), while the trend was totally opposite in population with severe CCVDs (T2DM OR=1.29 [1.00, 1.66]) with significant subgroup differences.
Conclusion
Our updated meta-analysis demonstrated that ALDH2 rs671 GG populations had significantly higher levels of BMI, blood pressure, FBG, TG, LDL-C and higher risk of hypertension than GA/AA populations. Besides, to the best of our knowledge, we first report GG had a higher risk of T2DM in population without severe CCVDs, and GA/AA had a higher risk of T2DM in population with severe CCVDs.
Systematic Review Registration
https://www.crd.york.ac.uk/PROSPERO, identifier CRD42023389242.
Keywords: ALDH2, meta-analysis, cardiometabolic risk factor, T2DM, hypertension
Introduction
Acetaldehyde dehydrogenase 2 (ALDH2) in humans is a 517-amino acid polypeptide encoded by a nuclear gene located at chromosome 12q24 (1). Among the 19 kinds of human ALDH isozymes, ALDH2 is the most efficient Isozyme for metabolizing ethanol-derived acetaldehyde (2). Although ALDH2 is well-known for its crucial role in ethanol metabolism, ALDH2 also has other diverse pathophysiological effects: ALDH2 could also metabolize many other short-chain fatty aldehydes and some aromatic and polycyclic aldehydes, which provides essential protective enzyme functions against these toxic substances and potential mechanism of participating in the development of cardiovascular disease (CVD) (3, 4). Besides, recent research has revealed non-enzymatic functions of ALDH2, participating in lipid metabolism in hepatocytes, regulating foam cell formation in macrophages, and modulating cellular senescence in endothelial cells and vascular smooth muscle cells (5). ALDH2 A carriers have low ALDH2 enzyme activity, characterized by nausea, facial flushing, headache, palpitations and dizziness after drinking. This alcohol-induced ALDH2 A individual flushing syndrome is caused by a single G to A nucleotide change (also known as SNP rs671 G>A), substituting the glutamate to lysine at position 487 (see Supplementary Figure S1 ) (6). The ALDH2 A mutation has a dominant effect on the ALDH2 G allele, so the enzyme activity of homozygous ALDH2 AA is expected to be 1-4% enzyme activity of the wild-type, and enzyme activity of heterozygous ALDH2 GA is significantly lower than that of the wild-type by 50% (3).
CVD is a non-communicable disease responsible for a large number of deaths worldwide, and genetic factors have been proven to be one of the main risk factors (7, 8). Besides, Asians are more susceptible to metabolic disorders than other racial groups, regardless of obesity (9). However, only a limited number of specific genetic loci variations have been found between individuals of European and East Asian ancestry. Recently, several previously unreported variants among East Asian individuals had been reported related to type 2 diabetes (T2DM) in a comprehensive meta-analysis, which included 433,540 East Asian subjects from genome-wide association studies (GWAS) (10). Of these variants, ALDH2 rs671, which was estimated to be present in about 30–50% of the East Asian population, compared to less than 5% of the European population, was found to be most prominently associated with T2DM (11, 12).
Cardiometabolic risk factors encompass lifestyle habits and health conditions, such as obesity, dyslipidemia, hypertension, diabetes, and hyperuricemia (13). The interaction among these various cardiometabolic risk factors ultimately leads to the progression of cardiovascular diseases. In China, more than 40% of deaths are attributable to CVDs, and the number of CVD deaths has almost doubled in the past decades (14). The increasing prevalence of cardiometabolic risk factors underlies the rise of CVDs, and thus curbing the rising cardiometabolic pandemic is imperative (13). ALDH2 gene polymorphism had been widely studied in many human diseases (5, 9, 15, 16), but only a few research discussed the relationship between ALDH2 and cardiometabolic risk factors. One meta-analysis (Li et al., 2017) discussed the correlation between ALDH2 and T2DM with data from only six studies (17). Although it resulted in a significant conclusion, the sensitivity analysis and Egger’s test were positive, and the results between subgroups demonstrated an opposite trend of odds ratio (OR) value. In addition, the conclusions of several recently published articles were inconsistent with the meta-analysis (18, 19). Besides, the most recent meta-analysis discussing ALDH2 and hypertension (Zheng et. al., 2020) did not conduct any subgroup analysis (20). Meanwhile, ALDH2 was also reported in previous GWAS to be associated with hypertension, triglyceride (TG) and body mass index (BMI) (21). This revealed the prominent role of ALDH2 in the development of CVDs among Asians. Therefore, the meta-analysis to investigate the relationship between ALDH2 polymorphism and cardiometabolic risk factors in East Asian population needed to be updated.
Methods
Search strategy
Our protocol was prospectively registered in the International prospective register of systematic reviews (PROSPERO) registry (CRD42023389242). We followed the guidelines of Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) ( Supplementary Table S1 ). Two independent reviewers (RL and PM) searched PubMed, Web of Science, and Embase (all updated to Oct 30th, 2023) and screened the titles and abstracts for potential included studies. The search strategy is available as Supplementary Material ( Supplementary Table S2 ). We only full-text reviewed articles which met the inclusion criteria, and the reference lists of eligible articles were searched for additional citations.
Selection criteria
There was no restriction on age, sex, and language. The inclusion criteria were (1): studies included subgroup data of ALDH2 gene rs671 polymorphism (GG, GA, AA type). (2) studies included at least one of the cardiometabolic risk factors: BMI, Hypertension, systolic blood pressure (SBP), diastolic blood pressure (DBP), T2DM, fasting blood glucose (FBG), postprandial blood glucose (PPG), hemoglobin A1c (HbA1c), TG, total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C). (3) studies only included the East Asian population. We excluded studies discussing other single nucleotide polymorphisms of ALDH2.
Data extraction
Two researchers (RL and KQ) independently extracted general characteristics (number of participants, age, sex) and outcomes of cardiometabolic risk factors from eligible articles. Decisions were made by consulting another reviewer LC, when RL and KQ met disagreements and failed to reach a consensus. We emailed the corresponding authors for additional information when the data was incomplete.
Quality assessment
Newcastle Ottawa Scale (NOS) was used for quality assessment of cohort and case-control studies (22), and the Agency for Healthcare Research and Quality (AHRQ) was used for cross-sectional studies (23). Two researchers (JZ and TZ) independently assessed the quality of included studies. The decision will be reached by consulting a third reviewer LC, when met disagreements and failed to reach a consensus. The precise Hardy Weinberg Equilibrium (HWE) was used to test the distribution of genotypes and evaluated by chi-square tests. High quality is considered NOS ≥6 points or AHRQ ≥8 points, and PHWE>0.05.
Subgroup analysis
Several types of subgroup analysis were conducted in our study: (1) quality of study; (2) publication type (cross-sectional, cohort, case-control); (3) nationality (Chinese, Korean, Japanese); (4) population (with or without severe cardiac-cerebral vascular diseases (CCVDs), which including myocardial infarct, coronary artery disease, ischemic stroke and hemorrhagic stroke).
Statistical analysis
Revman 5.3 and Stata 16.0 software were used to analyze Meta-analysis data. All forest plots and funnel plots were produced by Revman 5.3 software. Continuous data would be calculated by mean difference (MD) with 95% confidence intervals (CI). Dichotomous data would be calculated by OR with 95% CIs. Heterogeneity in the result of the meta-analysis was assessed by Cochrane Q and I2 statistics. I2 ≤ 50% demonstrated a low heterogeneity, and the fixed-effect model would be used for analysis; I2>50% demonstrated a high heterogeneity, and the random-effect model would be used for analysis. All statistical tests were two-tailed, and P ≤ 0.05 was regarded as a statistically significant difference. Publication bias was assessed by funnel plots and Egger’s tests. Sensitivity analysis was performed in the meta-analysis by excluding each study once at a time to check whether the effectiveness of the outcome was determined by individual studies.
Result
Characteristics of meta-analysis
The detailed steps of the literature search are shown in Figure 1 : 373 studies were reviewed from three databases, and eight studies were reviewed through the references of eligible full-text articles; 263 studies were excluded after screening titles and abstracts, and the remaining 118 studies were reviewed in full text. After excluding 72 studies according to the selection criteria, 46 studies with, 54068 GG participants and, 36820 GA/AA participants were included in our meta-analysis (19, 20, 24–67).
Figure 1.
Flowchart of selection of studies for inclusion in our meta-analysis. (*Outcomes from Embase and Web of science were not included studies from Medline).
The general characteristics of included studies in our meta-analysis are shown in Table 1 . 23 studies were Chinese, 22 were Japanese, and one was Korean. As for publication type, 17 were cross-sectional studies, 13 were cohort studies, and 16 were case-control studies. Among all 46 studies, 36 studies (78.3%) included populations without severe CCVDs, and 35 studies (76.1%) were assessed as high quality according to HWE and NOS or AHRQ. Detailed procedures of NOS and AHRQ are shown in Supplementary Table S3 .
Table 1.
General characteristics of included studies in our meta-analysis.
Year Authora | Nationality | Populationc | Article type | Gender (M/F) | GG | GA | AA | F(G) | F(A) | HWE | NOS/AHRQ |
---|---|---|---|---|---|---|---|---|---|---|---|
2023 Hisamatsu | Japanese | Normal | Cross-sectional | All Male | 369 | 255 | 58 | 0.728 | 0.272 | 0.345 | 11 |
2023 Hyashida | Japanese | Normal | Cross-sectional | 4572/6338 | 6131 | 4779 | / | N/A | 9 | ||
2023 Lan | Chinese | EH | Case-control | 3057/1474 | 2123 | 2001 | 407 | 0.689 | 0.311 | 0.111 | 9 |
2023 Okura | Japanese | Normal | Cross-sectional | 41/30 | 38 | 30 | 3 | 0.746 | 0.254 | 0.618 | 8 |
2023 Zhang | Chinese | HSd | Case-control | 214/115 | 184 | 123 | 22 | 0.746 | 0.254 | 0.973 | 7 |
2022 Wub | Chinese | EH | Case-control | 2040/1017 | 1442 | 1358 | 257 | 0.694 | 0.306 | 0.042 | 9 |
2022 Li | Chinese | Normal | Prospective cohort | 3468/2700 | 3105 | 3063 | N/A | 8 | |||
2021 He | Chinese | Normal | Prospective cohort | 1110/1390 | 1571 | 801 | 128 | 0.789 | 0.211 | 0.134 | 8 |
2021 Ishida | Japanese | MId | Retrospective cohort | 158/60 | 98 | 106 | 14 | 0.693 | 0.307 | 0.111 | 6 |
2021 Kogiso | Japanese | NAFLD | Case-control | 161/153 | 160 | 154 | N/A | 6 | |||
2021 Takeno | Japanese | Normal | Prospective cohort | All Male | 53 | 35 | 6 | 0.750 | 0.250 | 0.998 | 6 |
2021 Zhu | Chinese | MId | Case-control | 81/51 | 273 | 263 | 64 | 0.674 | 0.326 | 0.998 | 7 |
2020 Hou | Chinese | MId | Retrospective cohort | 637/326 | 502 | 370 | 91 | 0.713 | 0.287 | 0.172 | 7 |
2020 Kim | Korean | Normal | Retrospective cohort | 3319/5207 | 5880 | 2424 | 222 | 0.832 | 0.168 | 0.332 | 7 |
2020 Wangb | Chinese | Normal | Cross-sectional | 8431/4670 | 8891 | 3857 | 353 | 0.826 | 0.174 | 0.027 | 9 |
2020 Zhu | Chinese | Normal | Cross-sectional | 250/299 | 322 | 227 | N/A | 9 | |||
2019 Hanb | Chinese | Normal | Cross-sectional | All Male | 376 | 80 | 11 | 0.891 | 0.109 | 0.036 | 7 |
2019 Xiab | Chinese | Normal | Cross-sectional | 389/224 | 379 | 213 | 21 | 0.792 | 0.208 | 0.400 | 7 |
2017 Li | Chinese | EH | Case-control | 1420/1798 | 1791 | 1090 | 157 | 0.768 | 0.232 | 0.820 | 8 |
2017 Ma | Chinese | Normal | Cross-sectional | 2805/1213 | 2759 | 1152 | 107 | 0.830 | 0.170 | 0.596 | 10 |
2017 Zhu | Chinese | MetS | Case-control | N/A | 2003 | 2292 | N/A | 9 | |||
2016 Mizunob | Japanese | MId | Retrospective cohort | 156/46 | 99 | 86 | 17 | 0.703 | 0.297 | 0.962 | 5 |
2016 Oniki | Japanese | Normal | Retrospective cohort | 196/145 | 202 | 118 | 21 | 0.765 | 0.235 | 0.797 | 8 |
2016 Sungb | Chinese | ISd | Case-control | All Male | 308 | 223 | 67 | 0.702 | 0.298 | 0.028 | 7 |
2016 Yin | Japanese | Normal | Cross-sectional | 907/911 | 1046 | 648 | 124 | 0.754 | 0.246 | 0.229 | 10 |
2015 Idewaki | Japanese | Normal | Cross-sectional | 2483/1917 | 2402 | 1655 | 343 | 0.734 | 0.266 | 0.051 | 8 |
2015 Qu | Chinese | ISd | Retrospective cohort | 111/48 | 100 | 52 | 7 | 0.792 | 0.208 | 0.997 | 7 |
2015 Taylor | Chinese | T2DM | Case-control | 1352/2400 | 2202 | 1334 | 216 | 0.765 | 0.235 | 0.759 | 8 |
2015 Yokoyma | Japanese | Normal | Cross-sectional | All Male | 1528 | 278 | / | N/A | 9 | ||
2013 Lv | Chinese | EH | Case-control | 300/165 | 282 | 169 | 14 | 0.788 | 0.212 | 0.162 | 7 |
2013 Morita | Japanese | DR | Case-control | 156/78 | 143 | 78 | 13 | 0.778 | 0.222 | 0.861 | 7 |
2013 Wang | Chinese | EH | Case-control | 1266/853 | 1228 | 769 | 122 | 0.761 | 0.239 | 0.994 | 8 |
2013 Yokoyama | Japanese | Normal | Cross-sectional | All Male | 1605 | 297 | N/A | 8 | |||
2012 Feng | Chinese | EH | Case-control | 91/20 | 53 | 24 | 3 | 0.812 | 0.188 | 0.991 | 7 |
2011 Hasi | Chinese | EH | Case-control | 81/80 | 138 | 23 | 0 | 0.929 | 0.071 | 0.621 | 7 |
2010 Xu | Chinese | CADd | Case-control | 157/152 | 516 | 306 | 29 | 0.786 | 0.214 | 0.129 | 7 |
2008 Dakeishi | Japanese | Normal | Cross-sectional | 492/183 | 519 | 143 | 13 | 0.875 | 0.125 | 0.696 | 9 |
2007 Hui | Japanese | EH | Case-control | 352/180 | 302 | 195 | 35 | 0.751 | 0.249 | 0.898 | 7 |
2007 Nagawasab | Japanese | Normal | Cross-sectional | 159/217 | 237 | 133 | 6 | 0.807 | 0.193 | 0.030 | 10 |
2007 Xu | Chinese | All CADd | Cross-sectional | 186/45 | 145 | 77 | 9 | 0.794 | 0.206 | 0.953 | 8 |
2004 Suzukib | Japanese | Normal | Retrospective cohort | 114/44 | 85 | 73 | N/A | 4 | |||
2003 Saito | Japanese | Normal | Cross-sectional | All Male | 177 | 137 | 21 | 0.733 | 0.267 | 0.719 | 8 |
2002 Amamoto | Japanese | Normal | Cross-sectional | 749/1286 | 1056 | 906 | 150 | 0.714 | 0.286 | 0.061 | 8 |
2002 Takagib | Japanese | All MId | Retrospective cohort | All Male | 1035 | 925 | 202 | 0.693 | 0.307 | 0.975 | 5 |
2000 Muratab | Japanese | Normal | Retrospective cohort | All Male | 90 | 63 | 10 | 0.745 | 0.255 | 0.973 | 4 |
1996 Suzukib | Japanese | Normal | Retrospective cohort | 127/85 | 120 | 80 | 12 | 0.755 | 0.245 | 0.962 | 5 |
N/A, not applicable; HWE, Hardy Weinberg Equilibrium; NOS, Newcastle Ottawa Scale; EH ,essential hypertension; MI, myocardial infarct; CAD, coronary disease; IS, ischemic stroke; HS, hemorrhagic stroke; MetS, metabolic syndrome; NAFLD, nonalcoholic fatty liver disease; DR, diabetic retinopathy; AHRQ, Agency for Healthcare Research and Quality; F(G), frequency of G gene. aSearch result updated to Oct 30th, 2023. bThese studies meet one of the criteria: (1) P(HWE)≤0.05; (2) assessed as low quality of NOS or AHRQ. We compare the forest plot with vs. without these studies, the significance of P values are the same, so they are included in our meta-analysis. cNormal population means without severe CCVDs in their included participants. dThese populations are regarded as participants with severe CCVDs, and they are separated as a subgroup in the meta-analysis.
Outcomes of meta-analysis
Summary findings of outcomes are shown in Table 2 . Firstly, the differences of all confounders in our study were insignificant. It was worth mentioning that ALDH2 had a significantly different effect on T2DM, so the Pgender>0.05 could eliminate the bias caused by gender. As for outcomes, the GG population had demonstrated a significantly higher BMI level than the GA/AA population (MD=-0.26 [-0.32, -0.19], P<0.001, detailed forest plot see Figure 2 ). Besides, all outcomes related to blood pressure revealed significant results (incidence of hypertension OR=0.83 [0.80, 0.86], P<0.001; SBP MD=-1.48 [-1.82, -1.14], P<0.001; DBP MD=-1.09 [-1.58, -0.61], P<0.001; detailed forest plot of hypertension see Figure 3 ). However, only FBG showed a significant difference (MD=-0.10 [-0.13, -0.07], P<0.001), while the incidence of T2DM resulted in an insignificant difference (detailed forest plot see Figure 4 ). Moreover, the lipid resulted significantly in some of the outcomes (TG MD=-0.07 [-0.09, -0.04], P<0.001; LDL-C MD=-0.04 [-0.05, -0.02]). In all, ALDH2 is significantly associated with the most cardiometabolic risk factors in the total population. All detailed forest plots are displayed in Supplemental Files (see Supplementary Figures S2 - S12 ). Funnel plots of BMI, hypertension and T2DM are also showed in Supplementary Figures S13 - 15 . Egger’s test was only significant in the outcome of HbA1c. All outcomes of the sensitivity analysis were also insignificant.
Table 2.
Summary findings of primary outcomes.
Characteristic | No. of study | Participants | Statistical method | 95% CI | I2 (%)a | P value | Egger's test |
---|---|---|---|---|---|---|---|
Confounders | |||||||
Age | 23 | 42614 | MD | 0.29 [-0.09, 0.67] | 66 | 0.13 | 0.139 |
Gender | 21 | 57139 | OR | 1.04 [0.96, 1.14] | 72 | 0.32 | 0.086 |
Smoker | 22 | 39990 | OR | 1.01 [0.92, 1.10] | 55 | 0.86 | 0.925 |
Outcomes | |||||||
BMI | 22 | 45455 | MD | -0.26 [-0.32, -0.19] | 0 | <0.001* | 0.103 |
Hypertention | 29 | 68446 | OR | 0.83 [0.80, 0.86] | 46 | <0.001* | 0.211 |
SBP | 17 | 46748 | MD | -1.48 [-1.82, -1.14] | 48 | <0.001* | 0.782 |
DBP | 17 | 46748 | MD | -1.09 [-1.58, -0.61] | 71 | <0.001* | 0.331 |
T2DM | 20 | 51429 | OR | 1.03 [0.89, 1.18] | 80 | 0.72 | 0.273 |
FBG | 19 | 35154 | MD | -0.10 [-0.13, -0.07] | 46 | <0.001* | 0.666 |
HbA1c | 10 | 6635 | MD | 0.03 [-0.01, 0.07] | 0 | 0.19 | 0.018* |
TC | 16 | 36984 | MD | -0.02 [-0.04, 0.00] | 37 | 0.08 | 0.869 |
TG | 20 | 43419 | MD | -0.07 [-0.09, -0.04] | 54 | <0.001* | 0.509 |
LDL-C | 17 | 37961 | MD | -0.04 [-0.05, -0.02] | 0 | <0.001* | 0.834 |
HDL-C | 19 | 42737 | MD | -0.02 [-0.04, 0.01] | 91 | 0.25 | 0.826 |
BMI, body mass index; SBP, systolic pressure; DBP, diastolic pressure;T2DM, type 2 diabetes mellitus; FBG, fast blood glucose; HbA1c, glycosylated haemoglobin A1c; TC, total cholesterol; TG, triglyceride; LDL-C, low-density lipoprotein cholesterol; HDL-C, high-density lipoprotein cholesterol; MD, mean difference; OR, odds ratio; CI, confidence interval. aI2≤50% used fixed-effect model for analysis; I2>50% used random-effect model for analysis. *P≤0.05.
Figure 2.
Forest plot of ALDH2 GA/AA vs. GG on BMI.
Figure 3.
Forest plot of ALDH2 GA/AA vs. GG on incidence of hypertension.
Figure 4.
Forest plot of ALDH2 GA/AA vs. GG on incidence of T2DM.
Subgroup analysis
There were four types of subgroup analyses in our study. Supplementary Table S4 shows that all subgroup differences between different qualities of included studies were insignificant, so the studies of low qualities were not excluded from our meta-analysis. Supplementary Table S5 shows that all subgroup differences between different nationalities of included studies were also insignificant. Besides, Supplementary Table S6 demonstrates that all subgroup differences between different article types were insignificant. However, we found that in populations without severe CCVDs, GG demonstrated a significantly higher incidence of T2DM and FBG level (GA/AA vs. GG: T2DM OR=0.88 [0.79, 0.97]; MD=-0.11 [-0.14, -0.08]), while the trend was totally opposite in population with severe CCVDs (GA/AA vs. GG: OR=1.29 [1.00, 1.66]; MD=0.25 [0.09, 0.41]) with significant subgroup differences (PT2DM=0.006, PFBG<0.001, detailed data see Supplementary Table S7 ). Moreover, other outcomes revealed insignificant subgroup differences. It is worth mentioning that the subgroup analysis between severe CCVDs and without severe CCVDs was the first time reported.
Discussion
Our meta-analysis indicated that ALDH2 rs671 GG gene type was not only a risk factor for promoting the development of hypertension, but also in increasing BMI, blood pressure, FBG, TG, and LDL-C. In addition, the impact of ALDH2 on T2DM and FBG varies significantly among different populations. In the population without severe CCVDs, the incidence of T2DM and FBG levels in the GG population were significantly higher than the GA/AA population. In contrast, the conclusion was totally opposite in the population with severe CCVDs. Our results were more comprehensive and convincing based on the following aspects: Firstly, our meta-analysis included more eligible studies, providing sufficient statistical efficacy. Secondly, confounding factors were examined in our meta-analysis to avoid the potential intervention. Thirdly, we conducted four subgroup analyses on factors that may affect the reliability to reduce the heterogeneity between different studies. Fourthly, sensitivity analysis and Egger’s test showed that the results were stable and reliable, and no significant publication bias was found.
Currently, there is no meta-analysis discussing the relationship between ALDH2 and BMI, and our meta-analysis demonstrated that ALDH2 GG populations had a significantly higher BMI than GA/AA populations in the outcome of all subgroups. Some clinical articles have reported lower BMI due to healthier dietary habits caused by flushing syndrome in the GA/AA population (68, 69). Besides, it should be clarified that the correlation between ALDH2 and BMI varies among different genders. Gender differences also exist in other cardiometabolic outcomes (10, 70). Several studies have reported the activation effect of estradiol on ALDH2 (71, 72). Besides, we cannot ignore the differences in alcohol consumption between different genders due to social factors in East Asian culture, as well as the protective effect of estrogen itself on insulin resistance (73).
T2DM and hypertension are common microvascular and macrovascular diseases in East Asian populations (74). ALDH2 mutants have lower levels of BMI, providing a partial explanation for the correlation between ALDH2 polymorphism and T2DM and hypertension. In addition, other literature also discussed the mechanisms between ALDH2 and cardiometabolic risk factors. Previous studies on ALDH2 rs671 polymorphism and T2DM had provided controversial results (25, 35, 38, 40, 57, 63, 67). This may be due to the sample size of most ALDH2 studies being relatively small (28 included studies of our study had participants less than, 1000), and ALDH2 was interfered with other confounding factors. Our study included abundant studies and analyzed the confounders (age, gender, and smoker) to avoid the potential bias. Besides, it was worth mentioning that ALDH2 rs671 polymorphism was reported to modify the association between dietary behaviors and BMI independently of drinking habits. Therefore, lower BMI and alcohol intake of GA/AA individuals were supposed to be the mechanism of ALDH2 on T2DM in populations without severe CCVDs. However, people with severe CCVDs revealed a higher risk of T2DM and FPG levels in the GA/AA population. Some mechanisms may explain it (1): Tan et al. demonstrated that ALDH2 alleviated the ischemia and reperfusion injury in diabetic cardiomyopathy through inhibition of mitoPTP opening and activation of PI3K/AKT/mTOR pathway (75); (2) low ALDH2 activity exacerbated 4HNE-mediated coronary endothelial cell injury and thereby cardiac dysfunction and ischemia-reperfusion injury (76, 77). A meta-analysis by Xu et al. also concluded the correlation between the higher risk of ischemic stroke and ALDH2 rs671-variant, especially in AA populations. Therefore, for CAD populations, GA/AA individuals had a higher risk of progressing to severe CCVDs, which explained the opposite outcomes in the severe CCVDs subgroup. Moreover, Chang et al. demonstrated that male Aldh2 knock-in mice were prone to develop glucose intolerance, insulin resistance, and fatty liver under diet-induced obesity. Proteomic analyses of the brown adipose tissue from the male Aldh2 knock-in mice identified increased 4-hydroxynonenal-adducted proteins involved in mitochondrial fatty acid oxidation and electron transport chain, leading to markedly decreased fatty acid oxidation rate and mitochondrial respiration. Similar phenomena were also reproduced in other studies of mice experiments (78, 79). In summary, we inferred that in the normal population, ALDH2 GG has a higher risk of T2DM due to higher BMI and alcohol consumption, but severe CCVDs contributed to exacerbating central muscle cell damage in the GA/AA individuals with a higher FBG level.
As for the hypertension-related results, a statistically significant correlation between ALDH2 and hypertension was observed in all subgroups. Therefore, we could confirm that the GG population had a higher risk of hypertension and higher levels of SBP and DBP as well. Although GA/AA individuals had more acetaldehyde accumulation, Zhang et al. reported in their cohort study that ethanol, rather than acetaldehyde, played a key role in alcohol-induced hypertension (80). Therefore, GA/AA individuals were protected from hypertension due to their lower ethanol intake than GG individuals. In addition, though our conclusion was consistent with the recently published meta-analysis by Zheng et al., we had updated hypertension-related data by adding from twelve new studies than their study (20). Moreover, we analyzed confounders (age and smoker) and three new subgroup analyses, which they did not include, so we believed our article was updated compared to previous studies.
ALDH2 also revealed a significant correlation with TG and LDL-C. TG is the primary dietary lipid, so the level of TG is almost only influenced by dietary habits (81). The higher TG level of GG populations demonstrated unhealthier dietary habits than GA/AA populations. In recent years, many researchers have tried to explain the differences between ALDH2 gene types and lipids in other mechanisms. Gibb et al. found that the ALDH2 rs671 mutant could repress the transcription of a lysosomal H+ pump subunit in nucleus, which is crucial for lipid degradation and foam cell formation (82). Besides, Zhong et al. also discovered an unexpected interaction of ALDH2 with the LDL receptor, which may directly act on ATP6V0E2 (a critical substance for maintaining lysosomal function and degradation of oxidized LDL-C) and increase foam cell formation (83). However, the correlation between ALDH2 and TG or LDL-C was not strong enough that not all outcomes were significant in the subgroups. Further studies of ALDH2 including lipid-related data were needed.
In all, ALDH2 is primarily involved in the degradation of acetaldehyde which reduced ALDH2 activity could lead to “Asian flush syndrome,” affecting alcohol intake. Additionally, ALDH2 could also metabolize endogenous lipid aldehydes. Reduced ALDH2 activity results in lipid aldehyde accumulation, generating reactive oxygen species and activating various oxidative stress pathways. Over the past few decades, numerous studies have shown the dual effect between ALDH2 gene polymorphisms and cardiovascular diseases: ALDH2 mutation could increase the risk of coronary artery disease, peripheral artery disease and stroke; ALDH2 mutation could also decrease the risk of hypertension and aortic aneurysms or dissections (5). Besides, recent research has revealed non-enzymatic functions of ALDH2, participating in lipid metabolism in hepatocytes, regulating foam cell formation in macrophages, and modulating cellular senescence in endothelial cells and vascular smooth muscle cells. Moreover, our meta-analysis concludes that ALDH2 mutants had lower BMI levels. In summary, ALDH2 has diverse pathophysiological effects, and ALDH2 mutations can variably influence cardiovascular metabolic risk factors through multiple pathways.
Our article included sufficient studies that there was no publication bias (Egger’s test P>0.05) in almost all outcomes. Besides, we first reported the significant subgroup differences in T2DM and FBG in severe CCVD subgroup analysis, which could explain the controversial conclusion in the previously published articles. However, there were several limitations in our study: (1) Meta-regression is unavailable to subgroup data from less than ten studies, leading to a low R-squared of regression model. Therefore, our article only analyzed confounding factors through subgroup analysis rather than adjusting the effect of alcohol consumption or BMI on outcomes of cardiometabolic risk factors. (2) We did not conduct the subgroup analysis of drinker/non-drinker, male/female, and different genotypes (for example, GG vs. AA) due to the insufficient (less than five studies) subgroup data in the included studies. Even though we analyzed these subgroup analyses, the heterogeneity was too high to obtain a credible conclusion. (3) Although we believed that alcohol consumption, dietary habits, and BMI were the critical factors between ALDH2 and cardiometabolic risk factors, due to the lack of original data of included studies, we could not conduct the mediating analysis and interaction effects in our study. Future studies were needed to confirm the cardiometabolic mechanism of ALDH2.
Conclusion
Our updated meta-analysis demonstrated that ALDH2 rs671 GG populations had significantly higher levels of BMI, blood pressure, FBG, TG, LDL-C and higher risk of hypertension than GA/AA populations. Besides, to the best of our knowledge, we first report GG had a higher risk of T2DM in population without severe CCVDs, and GA/AA had a higher risk of T2DM in population with severe CCVDs. Future studies were warranted to confirm the cardiometabolic mechanism of ALDH2.
Data availability statement
The original contributions presented in the study are included in the article/ Supplementary Material . Further inquiries can be directed to the corresponding author.
Author contributions
RL: Methodology, Project administration, Software, Writing – original draft. MP: Data curation, Funding acquisition, Investigation, Supervision, Writing – original draft. JZ: Data curation, Visualization, Writing – review & editing. KQ: Formal Analysis, Software, Visualization, Writing – review & editing. TZ: Formal Analysis, Supervision, Writing – review & editing. LC: Conceptualization, Supervision, Writing – review & editing.
Funding Statement
The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. This study was supported by the National Natural Science Foundation of China (LC, Grant no., 82170822; MP, Grant no., 81900734).
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Publisher’s note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
Supplementary material
The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fendo.2024.1333595/full#supplementary-material
Tetramer structure of ALDH2 enzyme
Forest plot of ALDH2 GA/AA vs. GG on Age
Forest plot of ALDH2 GA/AA vs. GG on Gender
Forest plot of ALDH2 GA/AA vs. GG on Smoker
Forest plot of ALDH2 GA/AA vs. GG on SBP
Forest plot of ALDH2 GA/AA vs. GG on DBP
Forest plot of ALDH2 GA/AA vs. GG on FBG
Forest plot of ALDH2 GA/AA vs. GG on HbA1c
Forest plot of ALDH2 GA/AA vs. GG on TC
Forest plot of ALDH2 GA/AA vs. GG on TG
Forest plot of ALDH2 GA/AA vs. GG on LDL-C
Forest plot of ALDH2 GA/AA vs. GG on HDL-C
Funnel plot of outcome of BMI
Funnel plot of outcome of Hypertension
Funnel plot of outcome of T2DM
PRISMA Checklist of our study
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Associated Data
This section collects any data citations, data availability statements, or supplementary materials included in this article.
Supplementary Materials
Tetramer structure of ALDH2 enzyme
Forest plot of ALDH2 GA/AA vs. GG on Age
Forest plot of ALDH2 GA/AA vs. GG on Gender
Forest plot of ALDH2 GA/AA vs. GG on Smoker
Forest plot of ALDH2 GA/AA vs. GG on SBP
Forest plot of ALDH2 GA/AA vs. GG on DBP
Forest plot of ALDH2 GA/AA vs. GG on FBG
Forest plot of ALDH2 GA/AA vs. GG on HbA1c
Forest plot of ALDH2 GA/AA vs. GG on TC
Forest plot of ALDH2 GA/AA vs. GG on TG
Forest plot of ALDH2 GA/AA vs. GG on LDL-C
Forest plot of ALDH2 GA/AA vs. GG on HDL-C
Funnel plot of outcome of BMI
Funnel plot of outcome of Hypertension
Funnel plot of outcome of T2DM
PRISMA Checklist of our study
Data Availability Statement
The original contributions presented in the study are included in the article/ Supplementary Material . Further inquiries can be directed to the corresponding author.