Figure 5.

The targeted approach is essentially an enrichment approach. (A) Untargeted approach for the protein 3D structure prediction supplemented by metagenome. (B) Targeted approach for the protein 3D structure prediction supplemented by metagenome. (C) Case studies of modeling Pfam PF07682 and PF05005 with MSA from different biomes as the untargeted approach. For each biome, the number of metagenome sequences and the proportion of aligned homologous sequences in all the metagenome sequences was calculated. The correctness of 3D structure models was determined by comparing them to the known structure, which was quantified using the TM‐score method. The MetaSource is a targeted approach that was developed in a prior study [70]. The model labeled with gray background color is the source biome predicted by MetaSource. In blue type, the model with the highest TM‐score is displayed. 3D, three‐dimensional; MSA, multiple sequence alignment