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. 2023 Oct 19;2(4):e143. doi: 10.1002/imt2.143

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© 2023 The Authors. iMeta published by John Wiley & Sons Australia, Ltd on behalf of iMeta Science.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Validation of two primer sets designed by multiPrime using two sequencing platforms. (A) Validation of the primer set on the ONT platform. MCS, mixed clinical specimens (2–4 clinical specimens in one tube); NC, negative control; stars indicate the target virus. (B) Validation of the primer set on the NextSeq. 500 platform. The primer set was designed by multiPrime (v2.0.3) with the following parameters: coordinate: 4; seq_number_ANI: 10; Others: Default. Clinical NC, normal clinical specimen, which refers to samples obtained from healthy individuals devoid of any viral sequences; NC, negative control refers to intentionally empty samples without any template; stars indicate the target virus.