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. 2023 Sep 28;2(3):317–327. doi: 10.1002/mlf2.12086

Figure 3.

Figure 3

The pVH1‐2‐VIR plasmid contributes to hypervirulence to clinical ST11 CRKP. (A) Growth curve of 1332 and 1332/pVH1‐2‐VIR (n = 3). (B) Relative fitness of pVH1‐2‐VIR plasmid carrying clones compared with pVH1‐2‐VIR‐free clones obtained by competition assays. Bars represent normalized relative fitness after subtracting the effect of pVH1‐2‐VIR (n = 3). (C) String length observed in tested CRKP isolates (n = 3). (D) Virulence potential of CRKP isolates tested in a Galleria mellonella infection model. Survival of mice infected with 5 × 105 CFU of each Klebsiella pneumoniae at 48 h is shown (n = 12). (E) Experimental setup for the intestinal colonization assay. (F) Colonization levels of CRKP isolates in feces at Days 1 to 5 after infection (n = 6). *p < 0.05; **p < 0.01; ***p < 0.001. CFU, colony‐forming unit; CRKP, carbapenem‐resistant K. pneumoniae; nd, not detected.