| Antibody |
Fgfr1 (rabbit monoclonal) |
Cell Signaling |
9740 |
(1:1000) |
| Antibody |
Fgfr2 (rabbit polyclonal) |
Santa Cruz |
sc122 |
(1:1000) |
| Antibody |
Collagen 2 (rabbit polyclonal) |
Cedarlane |
CL50241AP |
(1:1000) |
| Antibody |
Actin (mouse monoclonal) |
Cell Signaling |
3700 |
(1:1000) |
| Antibody |
Vinculin (rabbit monoclonal) |
Cell Signaling |
13901 |
(1:1000) |
| Antibody |
pERK (rabbit polyclonal) |
Cell Signaling |
9101 |
(1:1000) |
| Antibody |
Anti-Y653/654 (rabbit polyclonal) |
Cell Signaling |
3471S |
(1:1000) |
| Antibody |
Anti-pY766 FGFR1 (rabbit monoclonal) |
Cell Signaling |
2544S |
(1:1000) |
| Antibody |
Anti-pFRS2 (rabbit monoclonal) |
Cell Signaling |
3861S |
(1:1000) |
| Antibody |
Anti-pPLCγ (rabbit polyclonal) |
Cell Signaling |
2821S |
(1:1000) |
| Antibody |
Anti-PLCγ (rabbit polyclonal) |
Cell Signaling |
2822S |
(1:1000) |
| Antibody |
Anti-V5 (mouse monocolonal) |
Invitrogen |
46-0705 |
(1:1000) |
| Antibody (secondary) |
Anti-rabbit |
Promega |
W4011 |
(1:10,000) |
| Antibody (secondary) |
Anti-mouse |
Millipore Sigma |
A6782 |
(1:10,000) |
| Cell line (Homo sapiens) |
HEK 293T FGFR1 |
This paper |
|
Stable cell line developed and maintained by Hristova lab, identity authenticated by SRT profiling, negative for mycoplasma |
| Cell line (Rattus norvegicus) |
RCS WT |
PMID:749928
|
|
A gift from Benoit de Crombrugghe |
| Cell line (Rattus norvegicus) |
RCS Fgfr1-4 null |
PMID:33952673
|
|
|
| Cell line (Rattus norvegicus) |
RCSFgfr1
|
This paper |
|
Progenitors: Fgfr3/4 KO RCS cells from Carmine Settembre, identity authenticated by SRT profiling, negative for mycoplasma |
| Chemical compound, drug |
2× Laemmli Buffer |
Bio-Rad |
1610737 |
|
| Chemical compound, drug |
Tris/Gly/SDS running buffer |
Bio-Rad |
1610732 |
|
| Chemical compound, drug |
Transfer buffer |
Bio-Rad |
1610734 |
|
| Chemical compound, drug |
Fugene HD |
Promega |
E2311 |
|
| Commercial assay or kit |
Bio-Rad Mini-Protean TGX precast gels |
Bio-Rad |
4561026 |
|
| Commercial assay or kit |
PVDF membranes |
Bio-Rad |
1620177 |
|
| Commercial assay or kit |
West Femto Supersignal |
Thermo Fisher Scientific |
1706435 |
|
| Commercial assay or kit |
Bio-Rad Magic Red Caspase 3-7 kit |
Bio-Rad |
ICT 935 |
|
| Commercial assay or kit |
MTT Cell Proliferation Assay Kit |
Cell BioLabs |
CBS-252 |
|
| Other |
Iblot 2 Gel Transfer Device |
Thermo Fisher Scientific |
IB21001 |
Equipment for transfer |
| Gene (Rattus norvegicus) |
Fgfr1
|
Ensembl |
Ensembl:ENSRNOG00000016050 |
|
| Peptide, recombinant protein |
FGF4 |
R&D Systems |
235-F4-025 |
|
| Peptide, recombinant protein |
FGF8 |
R&D Systems |
423-F8-025 |
|
| Peptide, recombinant Protein |
FGF9 |
R&D Systems |
273-F9-025 |
|
| Recombinant DNA reagent |
FGFR1-ECTM-eYFP (plasmid) |
PMID:26725515
|
|
YFP-Dr M. Betenbaugh, FGFR1 in pRK5- Dr M Mohammadi, into pcDNA3.1 vector |
| Recombinant DNA reagent |
FGFR1-ECTM-mCherry (plasmid) |
PMID:26725515
|
|
pRSET-mCherry- Dr R.Tsien, FGFR1 in pRK5- Dr M Mohammadi, into pcDNA3.1 vector |
| Recombinant DNA reagent |
pKrox(MapERK)d1EGFP |
This paper |
Addgene plasmid #214912 |
Progenitors: pKrox24(MapErk)DsRed (PMID:28199182), pTR01F (PMID:24376882), d1EGFP (PMID:16508309), PCR of mEgr1 3’UTR |
| Software |
FIF software |
PMID:31110281
|
|
|
| Software |
Mathematica |
Wolfram |
13 |
|
| Software |
Prism |
GraphPad |
9.2.0 |
|
