Abstract
Correction for ‘Surface enhanced Raman scattering for the multiplexed detection of pathogenic microorganisms: towards point-of-use applications’ by Matthew E. Berry et al., Analyst, 2021, DOI: 10.1039/D1AN00865J.
The authors regret that an incorrect version of Table 1 was included in the original article. The correct version of Table 1 is presented below.
A summary of the general approaches and studies discussed within this review.
| SERS approach | Summary | Performance |
|---|---|---|
| (1) Label-free detection | • SERS substrates for the capture of pathogens. | • Detection of three Gram negative bacterial strains (LOD 105 CFU mL−1).50 |
| • Intrinsic vibrational fingerprint of pathogens detected (no Raman reporters used). | • Detection of E. coli and S. aureus in tap water and milk (LOD 103 CFU mL−1, 10 min).51 | |
| • Detection of multiple bacterial strains (LOD 1 CFU mL−1, 5 min).52 | ||
| • Detection of bacterial strains in mung bean sprouts (LOD 102 CFU mL−1, 4 hours).53 | ||
| • Sampling and detection of bacterial pathogens from skin wound (LOD 106 CFU mL−1, 8 hours (5 min for sampling)).99 | ||
| • Detection of meningitis pathogens in clinical CSF.103 | ||
| (2) Microfluidics | • Sample preparation, reaction, separation and detection integrated into a single device. | • Label-free detection of E. coli and S. aureus in blood (LOD 105 CFU mL−1, 100 CFU mL−1 in culture).57 |
| • Supports label-free and label-based detection. | • Label-free detection of three bacterial strains in serum (LOD 4 CFU mL−1, 15 min).59 | |
| • Label-based detection of S. enterica and N. lactamica using DEP enrichment (LOD 70 CFU mL−1, 10 min).61 | ||
| • Label-based detection of three bacterial pathogens at millilitre scale in blood (LOD <100 CFU mL−1, 13 min).63 | ||
| • Label-based detection of E. coli using DEP enrichment (LOD 1 CFU mL−1).65 | ||
| • Detection of eight foodborne pathogens.98 | ||
| • Label-free detection of multiple viral strains in clinical nasopharyngeal swabs.106 | ||
| (3) Nucleic acid-based detection | • Coupling of assays for the detection of pathogenic DNA with SERS substrates. | • Detection of meningitis pathogenic DNA (LOD in pM range).72 Detection of pathogen DNA in clinical CSF.73 |
| • SERS nanotags functionalised with DNA/RNA aptamers. | • Detection of KSHV and BA DNA using LFA (LOD in fM range).74 | |
| • Supports label-based detection. | • Detection of S. aureus and S. typhimurium using aptamer-based magnetic sandwich assay (LOD 15 CFU mL−1).78 | |
| • Detection of E. coli and S. aureus using aptamer-based magnetic sandwich assay in urine samples (LOD 50 CFU mL−1, 1.5 hours, 20 CFU mL−1 and 15 min for culture).79 | ||
| • Detection of E. coli using aptamers in beef samples (LOD 100 CFU mL−1, 20 min).81 | ||
| • Detection of influenzae A H1N1 in complex mixtures (LOD 97 pfu mL−1, 20 min).82 | ||
| • Detection of DNA from 11 common RTI pathogens using LFA (LOD in fM range, 20 min). Detection from throat swab.110 | ||
| • Detection of S. enterica and L. monocytogenes in milk, chicken and beef using LFA and RPA (LOD 22 CFU mL−1).111 | ||
| • Detection of plant pathogens on commercial crops using RPA outside of laboratory.114 | ||
| • Real time detection of MRSA genes using miniaturized PCR system.115 | ||
| (4) Immunoassays | • Specific binding of pathogen antigen and antibodies coupled with SERS nanotags. | • Detection of Zika and dengue biomarkers using dipstick immunoassay (LOD 0.72 ng mL−1).86 |
| • Supports label-based detection. | • Detection of multiple viral strains using magnetic LFA (LOD 10 pfu mL−1).89 Detection in blood, serum and sputum (LOD 105 pfu mL−1). | |
| • Detection of three bacterial pathogens using magnetic sandwich assay (LOD 10 CFU mL−1).90 Same LODs confirmed on portable system (1 hour).108 | ||
| • Detection of multiple viral and bacterial pathogens in serum using magnetic sandwich assay (LOD 10 pg mL−1).91 | ||
The Royal Society of Chemistry apologises for these errors and any consequent inconvenience to authors and readers.