Table 2.

Antibodies used in study

Antibody target	Company and catalog #	Dilution	Isotype	Protein type/Expected localization	Immunogen
PAX7	DSHB (PAX7)	1:5–1:10	Mouse IgG1	TF/Nucleus	Amino acids 418–427
SNAI2	Cell signaling, mAb9585	1:200	Rabbit IgG	TF/Nucleus	Recombinant human Slug protein
SOX9	Millipore Sigma, AB5535	1:500	Rabbit IgG	TF/Nucleus	Epitope: C-terminal KLH-conjugated linear peptide corresponding to the C-terminal sequence of human Sox9
ECAD	BD Transduction Laboratories 61081	1:500	Mouse IgG2a	Cell adhesion/Membrane	Amino acids 735–883
NCAD	DSHB (NCAD), MNCD2	1:5	Rat IgG1	Cell adhesion/Membrane	Chicken N-cadherin
TUBA4A	Sigma, T6199	1:250	Mouse IgG1	Cytoskeleton/Cytosol	C-terminal end of the α-tubulin isoform (amino acids 426–430)
TUBB2A	Abnova, H00007280-M03	1:250	Mouse IgG2a	Cytoskeleton/Cytosol	TUBB2A (full-length recombinant protein with GST tag
TUBB3	R&D Systems, MAB1195	1:250	Mouse IgG2a	Cytoskeleton/Cytosol	Rat brain-derived microtubules