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[Preprint]. 2024 Mar 29:2024.03.28.587234. [Version 1] doi: 10.1101/2024.03.28.587234

Figure 3. Concentration dependence of RPA-ssDNA complexes.

Figure 3.

A-H) FRET analysis of (dT)25 ssDNA bound to increasing concentrations of RPA show a shift from the unbound to bound complex. As RPA concentrations are increased, a complete shift to the bound population is observed. I-L) Mass photometry analysis of RPA and RPA-(dT)25 complexes show formation of predominantly single RPA bound (dT)25 complexes.