Figure 1. Thyroid hormone distalizes gene expression patterns during regeneration.

(A) Multidimensional scaling plot comparing gene expression profiles in different regions (proximal, 4 dpa; middle, 7 dpa; distal, 15 dpa) of regenerating tissue from WT and hypothyroid fish; each data point represents one biological replicate. (B) Volcano plot showing differential gene expression between regenerating proximal and distal regions in WT. Filled grey circles indicate thyroid hormone-dependent genes. (D, F) scpp7 relative expression in (C) WT and (F) hypothyroid tissue samples. Note increased proximal expression in hypothyroid distal tissues. Whole mount fluorescent in situ hybridization using custom scpp7 RNAscope probe on (D-E) WT and (G-H) hypothyroid tissue regenerating (D, G) proximal or (E, H) distal fin tissues. Warm colors indicate highest regions of expression. Scale bar, 200 μm