Table 2:
Example for qPCR plate set up. Plasmid standards are loaded in duplicate. The 10-fold diluted plasmid standard ranges from 2E+02 to 2E+08 molecules/μL. For each standard, 5μL are loaded for a range of 1E+03 to 1E+09 total molecules. For the AAV reference sample and all other AAV samples, 4 dilutions each are loaded in duplicate. A no template control (NTC) is included on the plate.
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | |
|---|---|---|---|---|---|---|---|---|
| A | 1.00E+09 | 1.00E+08 | 1.00E+07 | 1.00E+06 | 1.00E+05 | 1.00E+04 | 1.00E+03 | NTC |
| B | ||||||||
| C | AAV reference | Sample 3 | ||||||
| D | ||||||||
| E | Sample 1 | Sample 4 | ||||||
| F | ||||||||
| G | Sample 2 | Sample 5 | ||||||
| H | ||||||||