Table 1.

Evaluation of the differences in (i) activity loss and (ii) mortality of cercariae of four trematode taxa between temperatures (6 vs 13°C)

Trematode taxa	(i) Activity loss						(ii) Mortality
(N, number cercariae)	Time section	exp(β)a	se(β)b	z-valuec	P value	Interpretation of activity loss	Time section	exp(β)	se(β)	z-value	P value	Interpretation of mortality
Diplostomum spp. 6°C, N = 415 13°C, N = 298	0–9 h	0.581	0.137	−3.973	<0.001	1.72 times ▾	0–22 h	1.354	0.1015	2.985	0.003	1.35 times ▴
	9–22 h	2.764	0.207	4.916	<0.001	2.76 times ▴	22–76 h	1.673	0.2398	2.145	0.032	1.67 times ▴
	22–76 h	5.3506	0.307	5.459	<0.001	5.35 times ▴
Apatemon spp. 6°C, N = 215 13°C, N = 214	0–38 h	0.669	0.113	−3.558	<0.001	1.49 times ▾	0–68 h	7.888	0.1462	14.120	<0.001	7.89 times ▴
Small-sized Plagiorchis spp. 6°C, N = 426 13°C, N = 423	0–15 h	3.649	0.119	10.923	<0.001	3.65 times ▴	0–72 h	11.668	0.120	20.470	<0.001	11.67 times ▴
	15–20 h	0.505	0.177	−3.851	<0.001	1.98 times ▾
	20–48 h	0.594	0.262	−1.990	0.047	1.68 times ▾
Large-sized Plagiorchis spp. 6°C, N = 62 13°C, N = 65	0–33 h	1.510	0.373	1.105	0.269		0–44 h	1.641	0.2492	1.988	0.047	1.64 times ▴
	33–44 h	0.762	0.468	−0.582	0.561		44–86 h	0.347	0.3986	−2.653	0.008	2.88 times ▾
	44–70 h	0.299	0.564	−2.142	0.032	3.35 times ▾

Results evaluating (i) Cox proportional hazards regression (CoxPH) [time active cercariae – temperature + plate (random) + trial (random)], and (ii) Cox proportional hazards regression (CoxPH) [time live cercariae – temperature + plate (random) + trial (random)]. If step function was required to accomplish the model assumptions, selected time sections are indicated in the table. The interpretation of results, comparing 6°C to 13°C, uses arrows to indicate a higher (▴) or lower (▾) activity loss or mortality of cercariae at 13°C. Statistically significant results (at α = 0.05) are indicated in bold.

^aHazard ratio (exponentiated coefficients). The hazard rate of cercariae at 6°C in both models (i, ii) is 1, to which the other levels are compared. If exp^(β) < 1, activity loss or mortality risk is reduced for the trematode taxa at 13°C.

^bStandard error of coefficients.

^cTest criterion value.