Fig. 2.

Organoids transduced with shRNA for ST6GAL1 have decreased ST6GAL1 protein expression and are less resistant to apoptosis after chemoradiation. A. ST6GAL1 mRNA expression is decreased with targeted knockdown. B. ST6GAL1 protein is decreased with knock down and this persists even after chemoradiation. Representative western blot shown of organoid model. C. Representative images of one organoid model with Cleaved Caspase-3 staining for apoptosis. Images are 4x, scale bar 1mm. C. Representative analysis of 3 experiments of staining an organoid model for percent Cleaved Caspase-3. Minimum of 5 images were taken at high power (60X) for analysis. Standard deviation error bars. One-way ANOVA p < 0.05. CV control vector, KD ST6GAL1 knockdown organoid model.