Table 1.

Media compositions.

a. OPC proliferation media compositions.
Base supplements: 2 % B27 + 1 % GlutaMAX™ + 1 % Penicillin/Streptomycin
Base growth factors: 30 ng/mL PDGF-AA + 10 ng/mL FGF
Media composition
1	2	3	4	5	6
DMEM	DMEM + EGF	DMEM/F12	DMEM/F12+EGF	Neurobasal	Neurobasal + EGF
EGF concentration: 10 ng/mL

b. OL differentiation media compositions.
Base supplements: 2 % B27 + 1 % GlutaMAX™ + 1 % Penicillin/Streptomycin +1 % N2
Base growth factors: 40 ng/mL Triiodothyronine (T3)
Media composition
1	2	3	4	5	6
DMEM	DMEM + CNTF	DMEM/F12	DMEM/F12+CNTF	Neurobasal	Neurobasal + CNTF
CNTF concentration: 10 ng/mL