Abstract
Quantitative changes in major histocompatibility class I antigen expression in tumour cells are believed to affect the host immune response against the tumour. In tumourigenic (TGrIII) human urothelial cell lines the apparent loss of polymorphic HLA-A,B epitopes has previously been demonstrated. In the present study, 3 non-tumourigenic (TGrII) and 6 tumourigenic (TGrIII) human urothelial cell lines have been investigated for their quantitative expression of monomorphic HLA-A,B,C and B2-microglobulin. Evidence is provided that an inverse correlation exists between tumourigenicity and HLA-A,B,C and B2-microglobulin expression. Furthermore, treatment of the cells with neuraminidase partly restored the expression of monomorphic HLA-A,B,C suggesting that at least some of the observed quantitative differences could be due to masking of the membrane bound HLA antigens by sialic acid-containing glycoconjugates.
Keywords: Tumour Cell, Immune Response, Cancer Research, Inverse Correlation, Quantitative Difference
References
- 1.Debray H, Zhu Q, Delanny P, Montreuil J, Dus D, Radzikowski C, Christensen B, Kieler J. Altered glycosylation of membrane glycoproteins in human uroepithelial cell lines. Int J Cancer. 1986;37:602–612. doi: 10.1002/ijc.2910370421. [DOI] [PubMed] [Google Scholar]
- 2.Christensen B, Kieler J, Vilien M, Don M, Wang CY, Wolf R. A classification of human urothelial cells propagated in vitro. Anticancer Res. 1984;4:319–338. [PubMed] [Google Scholar]
- 3.Doherty PC, Knowles BB, Wettstein PJ. Immunological surveillance of tumours in the context of major histocompatibility complex restriction of T-cell function. Adv Cancer Res. 1984;42:1–65. doi: 10.1016/s0065-230x(08)60455-8. [DOI] [PubMed] [Google Scholar]
- 4.Doyle A, Martin WJ, Funa R, Gazdar A, Carney D, Martin SE, Linnoila I, Cuttitta F, Mulshine J, Bunn P, Minna J. Marked decreased expression of Class I histocompatibility antigens, proteins, and mRNA in human small-cell lung cancer. J Exp Med. 1985;161:1135–1151. doi: 10.1084/jem.161.5.1135. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 5.Festenstein H, Garrido F. MHC antigens and malignancy. Nature. 1986;322:502–503. doi: 10.1038/322502a0. [DOI] [PubMed] [Google Scholar]
- 6.Goodenow RS, Vogel JM, Linsk RL. Histocompatibility antigens on murine tumours. Science. 1985;230:777–783. doi: 10.1126/science.2997918. [DOI] [PubMed] [Google Scholar]
- 7.Hicks RM, Wakefield JSTJ. Membrane changes during urothelial hyperplasia and neoplasia. Cancer Res. 1976;36:2502–2507. [PubMed] [Google Scholar]
- 8.Ingh HF, Ruiter DJ, Griffioen G, Muijen GNP, Ferrone S. HLA antigens in colorectal tumours — low expression of HLA Class I antigens in mucinous colorectal carcinomas. Br J Cancer. 1987;55:125–130. doi: 10.1038/bjc.1987.26. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 9.Johnson GD, Nogueira Araujo GMC. A simple method of reducing fading of immunofluorescence during microscopy. J Immunol Methods. 1981;43:349–350. doi: 10.1016/0022-1759(81)90183-6. [DOI] [PubMed] [Google Scholar]
- 10.Kieler J. Invasiveness of transformed bladder epithelial cells. Cancer Metas Rev. 1984;3:265–296. doi: 10.1007/BF00048389. [DOI] [PubMed] [Google Scholar]
- 11.Lampson LA, Fisher CA, Whelan J. Striking paucity of HLA-A,B,C and B2-microglobulin on human neuroblastoma cell lines. J Immunol. 1983;130:2471–2478. [PubMed] [Google Scholar]
- 12.Momburg F, Degener T, Bacchus E, Moldenhauer G, Hammerling GJ, Moller P. Loss of HLA-A,B,C and de novo expression of HLA-D in colorectal cancer. Int J Cancer. 1986;37:179–184. doi: 10.1002/ijc.2910370203. [DOI] [PubMed] [Google Scholar]
- 13.Natali PG, Bigotti A, Nicotra MR, Viora M, Manfred D, Ferrone S. Distribution of human Class I (HLA-A,B,C) histocompatibility antigens in normal and malignant tissues of nonlymphoid origin. Cancer Res. 1984;44:4679–4687. [PubMed] [Google Scholar]
- 14.Ottesen SS, Kieler J, Christensen B. Changes in HLA-A,B,C expression during “spontaneous” transformation of human urothelial cells in vitro. Eur J Cancer Clin Oncol. 1987;23:991–995. doi: 10.1016/0277-5379(87)90347-6. [DOI] [PubMed] [Google Scholar]
- 15.Parham P, Barnstable CJ, Bodmer WF. Use of a monoclonal antibody (W6/32) in structural studies of HLA-A,B,C antigens. J Immunol. 1979;123:341–349. [PubMed] [Google Scholar]
- 16.Stroynowski I, Clark S, Henderson LA, Hood L, McMillan M, Forman J. Interaction of 01 with 02 region in Class I MHC proteins contributes determinants recognized by antibodies and cytotoxic T cells. J Immunol. 1985;135:2160–2166. [PubMed] [Google Scholar]
- 17.Trowssdale J, Travers P, Bodmer F, Patillo RA. Expression of HLA-A,B,C and B2-microglobulin antigens in human choriocarcinoma cell lines. J Exp Med. 1980;152:11s–17s. [PubMed] [Google Scholar]
- 18.Vilien M, Wolf H, Rasmussen F. Immunological characterization of cell lines established from malignant and normal human urothelium. Eur J Cancer. 1981;17:321–327. doi: 10.1016/0014-2964(81)90123-7. [DOI] [PubMed] [Google Scholar]
- 19.Vilien M, Christensen B, Wolf H, Rasmussen F, Hou-Jensen C, Povlsen CO. Comparative studies of normal, “spontaneously” transformed and malignant urothelium cells in vitro. Eur Cancer Clin Oncol. 1983;19:775–789. doi: 10.1016/0277-5379(83)90010-x. [DOI] [PubMed] [Google Scholar]
- 20.Walton GR, McCue PA, Graham SD., Jr Beta-2 microglobulin as a differentiation marker in bladder cancer. J Urol. 1986;136:1197–1200. doi: 10.1016/s0022-5347(17)45281-5. [DOI] [PubMed] [Google Scholar]
- 21.Yogeeswaran G, Salk PL. Metastatic potential is positively correlated with cell surface sialylation of cultured murine tumor cell lines. Science. 1981;212:1514–1516. doi: 10.1126/science.7233237. [DOI] [PubMed] [Google Scholar]