Skip to main content
PMC home page
Cancer Immunology, Immunotherapy : CII logoLink to Cancer Immunology, Immunotherapy : CII
. 1988 Sep;27(2):171–176. doi: 10.1007/BF00200023

The altered expression of MHC-class II determinants on monocytes of cancer patients

Wojciech Uracz 1, Jerzy Stachura 2, Anna Pituch-Noworolska 1, Tadeusz Popiela 3, Antoni Czupryna 3, Marek Zembala 1,✉
PMCID: PMC11038092  PMID: 2458182

Abstract

The expression of MHC class II determinants Ia.7 (detected by cross reactive mouse anti-Iak antibody) and HLA-DR on monocytes (MO) of gastric and colorectal cancer patients was examined. An increased proportion of MO bearing the Ia.7 determinant was found, while the number of MO expressing DR was not elevated. In gastric cancer patients the increased expression of the Ia.7 determinant was most pronounced in advanced cancer (stage IVA and IVB). The increased expression of this determinant was related to the presence of the tumour as the number of MO expressing Ia.7 decreased 6 months following surgical resection of the tumour. Further, the increased expression of Ia.7 on MO correlated with the tumour infiltration of the serosa. The Ia.7 determinants were mainly expressed on MO which also expressed the receptor for the Fc part of immunoglobulin. Immunostaining in cellular infiltrates surrounding the tumour revealed that Ia.7+ macrophages (MØ) were more numerous than in normal gastric mucosa and severe chronic gastritis and were mostly present in close proximity to tumour cells, while DR+ MØ were mainly localized within the stromal tissue of the tumour and their number was not increased in cancer infiltrates. These observations indicate that the Ia.7+ subpopulation of MØ may be involved in the anti-tumour response of the host.

Keywords: Cancer Patient, Gastric Cancer, Colorectal Cancer, Gastritis, Gastric Mucosa

References

  • 1.Allen C, Hogg N. Elevation of infiltrating mononuclear phagocytes in human colorectal tumors. J Natl Cancer Inst. 1987;78:465. [PubMed] [Google Scholar]
  • 2.Allen CA, Hogg N. Association of colorectal tumor epithelium expressing HLA-D/DR with CD8-positive T-cells and mononuclear phagocytes. Cancer Res. 1987;47:2919. [PubMed] [Google Scholar]
  • 3.Basham TY, Merigan TC. Recombinant interferon gamma increases HLA-DR synthesis and expression. J Immunol. 1983;130:1492. [PubMed] [Google Scholar]
  • 4.Baxevanis CN, Nagy ZA, Klein J. A novel type of T-T cell interaction removes the requirement for I-B region in the H-2 complex. Proc Natl Acad Sci USA. 1981;78:3809. doi: 10.1073/pnas.78.6.3809. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 5.Brown R, Wolman M. Differentiation of macrophages for Lewis lung carcinoma tumour cells in tissue sections by their alpha-naphthyl butyrate esterase activity. Histochem J. 1981;13:975. doi: 10.1007/BF01002637. [DOI] [PubMed] [Google Scholar]
  • 6.Cambell DA, Jr, Oehler JR, Tsai CT, LoBuglio AF, Niederhuber JE. Effect of mouse anti-I region antiserum and complement on human mononuclear cell response to concanavalin A. J Immunol. 1982;128:2057. [PubMed] [Google Scholar]
  • 7.Fielding JWL, Roginski C, Ellis DJ, Jones BG, Powell J, Waterhouse JA, Brookes VS. Clinicopathological staging of gastric cancer. Br J Surg. 1984;71:677. doi: 10.1002/bjs.1800710910. [DOI] [PubMed] [Google Scholar]
  • 8.Gehan EA. A generalized Wilcoxon test for comparing arbitrarily singly censored samples. Biometrica. 1965;52:203. [PubMed] [Google Scholar]
  • 9.Herman J, Kew MC, Rabson AR. Defective interleukin-1 production by monocytes from patients with malignant disease. Cancer Immunol Immunother. 1984;16:182. doi: 10.1007/BF00205426. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 10.Ikezawa Z, Nonaka M, Abe R, Tada T, Nagy ZA, Klein J. Induction of T cell responses in nonresponder mice by abolishing suppression with monoclonal antibodies recognizing A region-controlled, T cell specific determinants. J Immunol. 1983;131:1646. [PubMed] [Google Scholar]
  • 11.Janeway C, Bottomly K, Babich J, Conrad P, Conzen S, Jones B, Kye J, Katz M, McVay L, Murphy DB, Tite J. Quantitative variation in Ia antigen expression plays a central role in immune regulation. Immunol Today. 1984;5:99. doi: 10.1016/0167-5699(84)90043-4. [DOI] [PubMed] [Google Scholar]
  • 12.Kowalczyk D, Ruggiero I, Zembala M. Monocyte regulation of lymphokine production in cancer patients. J Clin Immunol. 1985;19:37. [PubMed] [Google Scholar]
  • 13.Mytar B, Zembala M, Uracz W, Czupryna A. Cytostatic activity on tumour cells of monocytes from patients with gastrointestinal cancer. Cancer Immunol Immunother. 1982;130:190. doi: 10.1007/BF00205387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 14.Nunez G, Giles RC, Ball I, Hurley CK, Capra JD, Stastny P. Expression of HLA-DR, MB, MT and SB antigens on human mononuclear cells: Identification of two phenotypically distinct monocyte subpopulations. J Immunol. 1984;133:1300. [PubMed] [Google Scholar]
  • 15.Ozato K, Mayer N, Sachs D. Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens. J Immunol. 1980;124:533. [PubMed] [Google Scholar]
  • 16.Rowe DJ, Beverley PCL. Characterization of breast cancer infiltrates using monoclonal antibodies to human leukocyte antigens. Br J Cancer. 1984;49:149. doi: 10.1038/bjc.1984.27. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 17.Siegiel S. Nonparametric statistic for the behavioral sciences. New York: Mc Graw-Hill; 1963. [Google Scholar]
  • 18.Schmitz-Moormann P, Heider HA, Thomas C. Prognosis independent of therapy. In: Herfarth CH, Schlag P, editors. Gastric cancer. Verlag, Berlin, Heidelberg, New York: Springer; 1979. p. 172. [Google Scholar]
  • 19.Sternberger LA, Sternberger NH. Principles of immunochemistry. In: Russo J, editor. Immunochemistry in tumour diagnosis. Boston, Dordrecgt, Lancaster: Martinus Nijhoff Publ; 1985. p. 12. [Google Scholar]
  • 20.Unanue ER. Antigen-presenting function of the macrophage. Annu Rev Immunol. 1983;2:395. doi: 10.1146/annurev.iy.02.040184.002143. [DOI] [PubMed] [Google Scholar]
  • 21.Uracz W, Mytar B, Zembala M, Ruggiero I, Popiela T, Czupryna A. Activated monocytes in gastric cancer patients. II. Suppressor and cytostatic activity in vitro. J Cancer Res Clin Oncol. 1982;104:307. doi: 10.1007/BF00406249. [DOI] [PubMed] [Google Scholar]
  • 22.Uracz W, Pituch-Noworolska A, Popiela T, Zembala M. The Fc receptors of normal and cancer patients monocytes. Immunobiology. 1982;163:450. doi: 10.1016/S0171-2985(82)80059-4. [DOI] [PubMed] [Google Scholar]
  • 23.Uracz W, Pituch-Noworolska A, Zembala M, Popiela T, Czupryna A. „Activated” monocytes in gastric cancer patients. I. An increased Fc receptor expression, antibody dependent cellular cytotoxicity and NBT reduction. J Cancer Res Clin Oncol. 1982;104:181. doi: 10.1007/BF00402066. [DOI] [PubMed] [Google Scholar]
  • 24.Whitwell HL, Hughes HPA, Moore M, Ahmed A. Expression of major histocompatibility antigens and leucocyte infiltration in benign and malignant human breast disease. Br J Cancer. 1984;49:161. doi: 10.1038/bjc.1984.28. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 25.Yamamoto K, Kumagai Y, Hiramatsu K, Okumura K, Tada T. I-A homologous antigen on human lymphocytes detected by a monoclonal antibody. Immunogenetics. 1983;17:101. doi: 10.1007/BF00364294. [DOI] [PubMed] [Google Scholar]
  • 26.Zembala M, Uracz W, Ruggiero I, Lemmel EM. Isolation of human monocytes by rosetting with antibody-coated human erythrocytes and isopycnic gradient centrifugation. Clin Exp Immunol. 1982;49:225. [PMC free article] [PubMed] [Google Scholar]
  • 27.Zembala M, Mytar B, Ruggiero I, Uracz W, Popiela T, Czupryna A. Suppressor cells and survival of patients with advanced gastric cancer. J Natl Cancer Inst. 1983;70:223. [PubMed] [Google Scholar]
  • 28.Zembala M, Uracz W, Ruggiero I, Mytar B, Pryjma J. Isolation and functional characteristics of FcR+ and FcR− human monocyte subsets. J Immunol. 1984;133:1293. [PubMed] [Google Scholar]

Articles from Cancer Immunology, Immunotherapy : CII are provided here courtesy of Springer

RESOURCES