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. 1985 Oct;20(2):129–136. doi: 10.1007/BF00205679

Presence of normal human cell surface antigens in plasma of athymic mice bearing a human colon carcinoma and in normal human plasma

Yossi Markson 1, David W Weiss 2, Ofra Weiss 2, Fanny Doljanski 1,✉
PMCID: PMC11038173  PMID: 3849977

Abstract

The mixed haemadsorption (MHA) method was employed for detection of several normal antigenic components on the surface of human colon carcinoma cells (HT-29). The antigens were expressed by cells in monolayer cultures and in suspensions prepared by monolayer trypsinization, and by cells of tumours growing progressively in athymic mice. The plasma of such animals bearing medium sized and large, non-necrotic tumours contained all the antigens, as determined by the radial diffusion immune haemolysis method (RDIH); the plasma of animals with small or large heavily necrotic tumours did not contain detectable amounts of any of the determinants. The half-life of the determinants in the circulation as extracellular entities was ca. 20 h. The same antigens, and fibronectin, were found to be ubiquitously represented in normal human plasma. It is proposed that the presence of membrane antigens in plasma is the result of physiological shedding of cell surface constituents by living cells.

Keywords: Cell Surface, Surface Antigen, Colon Carcinoma, Monolayer Culture, Detectable Amount

References

  • 1.Allison JP, Pelegrino MA, Ferrone S, Callahan GN, Reisfeld RA. Biologic and chemical characterization of HLA antigens in human serum. J Immunol. 1977;118:1004. [PubMed] [Google Scholar]
  • 2.Black PH. Shedding from the cell surface of normal and cancer cells. Adv Cancer Res. 1980;32:75. doi: 10.1016/s0065-230x(08)60361-9. [DOI] [PubMed] [Google Scholar]
  • 3.Boctor AM, Bystryn JC. Degradation of tumor-associated antigens shed by human melanoma cells in culture. Cancer Res. 1982;42:2121. [PubMed] [Google Scholar]
  • 4.Burchell J, Wang D, Taylor-Papadimitriou J. Detection of the tumor-associated antigens recognized by the monoclonal antibodies HMFG-1 and 2 in serum from patients with breast cancer. Int J Cancer. 1984;34:763. doi: 10.1002/ijc.2910340605. [DOI] [PubMed] [Google Scholar]
  • 5.Bystryn JC. Shedding and degradation of cell-surface macromolecules and melanoma-associated antigens by human melanoma. In: Reisfeld RA, Ferrone S, editors. Melanoma antigens and antibodies. New York: Plenum; 1982. p. 37. [Google Scholar]
  • 6.Ceriani RL, Sasaki M, Sussman H, Wara WM, Blank EW. Circulating human mammary epithelial antigens in breast cancer. Proc Natl Acad Sci USA. 1982;79:5420. doi: 10.1073/pnas.79.17.5420. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 7.Davies G, Grant AG, Duke D, Hermon-Taylor J. Antibody response of nude (RNU/RNU) and hairy (RNU/+) rats to circulating cell surface components from human pancreatic cancer xenografts. Br J Cancer. 1983;48:239. doi: 10.1038/bjc.1983.179. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 8.Doljanski F. Cell surface shedding. In: Horowitz MI, editor. The glycoconjugates. New York: Academic Press; 1982. p. 155. [Google Scholar]
  • 9.Evrin PE, Wibell L. The serum levels and urinary excretion of β2 microglobulin in apparently healthy subjects. Scand J Clin Lab Invest. 1972;29:69. doi: 10.3109/00365517209081057. [DOI] [PubMed] [Google Scholar]
  • 10.Fogh J, Fogh JM, Orfeo F. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J Natl Cancer Inst. 1977;59:221. doi: 10.1093/jnci/59.1.221. [DOI] [PubMed] [Google Scholar]
  • 11.Giacomini P, Veglia F, Cordiali Fei P, Rehle T, Natali PG, Ferrone S. Level of a membrane-bound high-molecularweight melanoma-associated antigen and a cytoplasmic melanoma-associated antigen in surgically removed tissues and in sera from patients with melanoma. Cancer Res. 1984;44:1281. [PubMed] [Google Scholar]
  • 12.Gupta RK, Morton DL. Studies of a melanoma tumor-associated antigen detected in the spent culture medium of a human melanoma cell line by allogeneic antibody. I. Purification and development of a radioimmunoassay. J Natl Cancer Inst. 1984;72:67. doi: 10.1093/jnci/72.1.67. [DOI] [PubMed] [Google Scholar]
  • 13.Hames BD. An introduction to polyacrylamide gel electrophoresis. In: Hames BD, Riekwood D, editors. Gel electrophoresis of proteins. Oxford: IRL Press Ltd; 1981. p. 1. [Google Scholar]
  • 14.Hearing VJ, Gersten DM, Marchalonis JJ. Shed proteins in malignant melanoma. Anticancer Res. 1981;1:313. [PubMed] [Google Scholar]
  • 15.Klug TL, Bast RC, Jr, Niloff JM, Knapp RC, Zurawski VR., Jr Monoclonal antibody immunoradiometric assay for an antigenic determinant (CA 125) associated with human epithelial ovarian carcinomas. Cancer Res. 1984;44:1048. [PubMed] [Google Scholar]
  • 16.Köhler G, Milstein C. Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion. Eur J Immunol. 1976;6:511. doi: 10.1002/eji.1830060713. [DOI] [PubMed] [Google Scholar]
  • 17.Martin KW, Halpern SE. Carcinoembryonic antigen production, secretion, and kinetics in BALB/c mice and a nude mouse-human tumor model. Cancer Res. 1984;44:5475. [PubMed] [Google Scholar]
  • 18.Molinaro GA, Maron E, Eby WC, Dray S. A general method for enumerating single cells secreting antigens: Albumin-secreting hepatocytes detected as plaque-forming cells. Eur J Immunol. 1975;5:771. doi: 10.1002/eji.1830051108. [DOI] [PubMed] [Google Scholar]
  • 19.Mollison PL. Blood transfusion in clinical medicine. 5th edn. Oxford: Blackwell Scientific Publ; 1972. p. 228. [Google Scholar]
  • 20.Mori T, Mori E, Sudo T, Kano K. Expression of heterophil Forssman antigen as glycoprotein on transformed rat cell lines: Shedding of the antigen from the cells. J Natl Cancer Inst. 1984;73:1179. [PubMed] [Google Scholar]
  • 21.Mosseson MW, Amrani DL. The structure and biological activities of plasma fibronectin. Blood. 1980;56:145. [PubMed] [Google Scholar]
  • 22.Plesser YM, Weiss DW, Doljanski F. Cell-surface shedding by fibroblasts in culture. Isr J Med Sci. 1980;16:519. [PubMed] [Google Scholar]
  • 23.Plesser YM, Weiss DW, Markson Y, Doljanski F. Expression and shedding of major histocompatibility complex product and blood group antigens by cells in monolayer cultures. Cell Immunol. 1980;51:414. doi: 10.1016/0008-8749(80)90273-7. [DOI] [PubMed] [Google Scholar]
  • 24.Plesser YM, Markson Y, Weiss DW, Brautbar C, Doljanski F. Shedding of histocompatibility and blood group antigenic determinants from human epithelial cells and fibroblasts in culture. Cell Mol Biol. 1983;29:227. [PubMed] [Google Scholar]
  • 25.Povleson CO, Rygaard J. Heterotransplantation of human adenocarcinomas of the colon and rectum to the mouse mutant nude. A study of nine consecutive transplantations. Acta Pathol Microbiol Immunol Scand [A] 1971;79:159. doi: 10.1111/j.1699-0463.1971.tb03324.x. [DOI] [PubMed] [Google Scholar]
  • 26.Price MR, Baldwin RW. Shedding of tumor cell surface antigens. In: Poste G, Nicolson GH, editors. Cell surface reviews, vol 3. Amsterdam: North Holland Publ; 1977. p. 423. [Google Scholar]
  • 27.Ross AH, Herlyn M, Ernst CS, Guerry D, Bennicelli J, Ghrist BFD, Atkinson B, Koprowski H. Immunoassay for melanoma-associated proteoglycan in the sera of patients using monoclonal and polyclonal antibodies. Cancer Res. 1984;44:4642. [PubMed] [Google Scholar]
  • 28.Sasaki M, Peterson JA, Wara WM, Ceriani RL. Human mammary epithelial antigens (HME-Ags) in the circulation of nude mice implanted with a breast tumor and non-breast tumors. Cancer. 1981;48:2204. doi: 10.1002/1097-0142(19811115)48:10<2204::aid-cncr2820481015>3.0.co;2-0. [DOI] [PubMed] [Google Scholar]
  • 29.Sulitzeanu D (1985) Human cancer associated antigens: Present status and implications for immunodiagnosis. Adv Cancer Res (in press) [DOI] [PubMed]
  • 30.Vuento M, Vaheri A. Purification of fibronectin from human plasma by affinity chromatography under non-denaturing conditions. Biochem J. 1979;183:331. doi: 10.1042/bj1830331. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 31.Weiss DW. Conference summation: Sessions on host defense mechanisms, immune assessment and surveillance, and new approaches to cancer therapy. Transplant Proc. 1984;16:545. [Google Scholar]

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