Skip to main content
NCBI home page
Cancer Immunology, Immunotherapy : CII logoLink to Cancer Immunology, Immunotherapy : CII
. 1988 Sep;27(2):133–136. doi: 10.1007/BF00200017

Indomethacin stimulation of macrophage cytostasis against MOPC-315 tumor cells is inhibited by both prostaglandin E2 and nordihydroguaiaretic acid, a lipoxygenase inhibitor

Graham R Elliott 1,✉, Corne Tak 1, Carin Pellens 1, Shlomo Ben-Efraim 3, Iven L Bonta 1
PMCID: PMC11038305  PMID: 2843283

Abstract

Indomethacin enhanced macrophage cytostasis against MOPC-315 tumor cells in vitro. The effect of indomethacin was inhibited by prostaglandin E2 and by the lipoxygenase inhibitor nordihydroguaiaretic acid. Prostaglandin E2 and nordihydroguaiaretic acid also inhibited indomethacin stimulation of macrophage thymidine incorporation. Indomethacin inhibited macrophage prostaglandin E2 formation and stimulated leukotriene B4 synthesis. Nordihydroguaiaretic acid inhibited leukotriene B4 production. Our data indicate that eicosanoids play a role in regulating macrophage cytostasis.

Keywords: Tumor Cell, Cancer Research, Prostaglandin, Indomethacin, Thymidine

References

  • 1.Docherty JC, Wilson TW. Indomethacin increases the formation of lipoxygenase products in calcium ionophore-stimulated human neutrophils. Biochem Biophys Res Commun. 1987;2:534. doi: 10.1016/0006-291x(87)90909-0. [DOI] [PubMed] [Google Scholar]
  • 2.Ham EA, Soderman DD, Zanetti MA, Dougherty HW, McCauley E, Keuhl FA. Inhibition by prostaglandins of leukotriene B4 release from activated neutrophiles. Proc Natl Acad Sci USA. 1983;80:4349. doi: 10.1073/pnas.80.14.4349. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 3.Kunkel SL, Chensue SW, Phan SH. Prostaglandins as endogenous mediators of interleukin 1 production. J Immunol. 1986;136:186. [PubMed] [Google Scholar]
  • 4.Lee KC, Wong M, McIntyre D. Characterization of macrophage subpopulations responsive to activation by endotoxin and lymphokines. J Immunol. 1981;126:2474. [PubMed] [Google Scholar]
  • 5.Ophir R, Ben-Efraim S, Bonta IL. Leukotriene D4 and indomethacin enhance additively the macrophage cytostatic activity in vitro towards MOPC-315 tumor cells. Int J Tissue React. 1987;9:189. [PubMed] [Google Scholar]
  • 6.Opitz HG, Niethammer D, Jackson RC, Lemke H, Huget R, Flad HD. Biochemical characterization of a factor released by macrophages. Cell Immunol. 1975;18:70. doi: 10.1016/0008-8749(75)90037-4. [DOI] [PubMed] [Google Scholar]
  • 7.Parnham MJ, Bittner C, Winkelmann J. Chemiluminescence from mouse resident macrophages: characterization and modulation by arachidonic acid metabolites. Immunopharmacology. 1983;5:277. doi: 10.1016/0162-3109(83)90044-9. [DOI] [PubMed] [Google Scholar]
  • 8.Rola-Pleszczynski M, Lemaire I. Leukotrienes augment interleukin 1 production by human monocytes. J Immunol. 1985;135:3958. [PubMed] [Google Scholar]
  • 9.Rola-Pleszczynski M, Gagnon L, Rudzinska M, Borgeat P, Sirois P. Human natural cytotoxic cell activity: enhancement by leukotrienes (LT A4, B4 and D4, but not by stereoisomers of LTB4 or HETES. Prostaglandins Leukotrienes Med. 1984;13:113. doi: 10.1016/0262-1746(84)90111-2. [DOI] [PubMed] [Google Scholar]
  • 10.Schenkelaars EJ, Bonta IL. Cyclooxygenase inhibitors promote the leukotriene C4-induced release of beta-glucuronidase from rat peritoneal macrophages: prostaglandin E2 suppresses. Int J Immunopharmacol. 1986;8:305. doi: 10.1016/0192-0561(86)90112-8. [DOI] [PubMed] [Google Scholar]
  • 11.Yui S, Yamazaki M. Induction of macrophage growth by lipids. J Immunol. 1986;136:1334. [PubMed] [Google Scholar]

Articles from Cancer Immunology, Immunotherapy : CII are provided here courtesy of Springer

RESOURCES