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. 1994 Jan;38(1):31–37. doi: 10.1007/BF01517167

Macrophage and dendritic cell infiltration in head and neck squamous-cell carcinoma; an immunohistochemical study

Jeroen D Kerrebijn 1,✉, Alphons J M Balm 3, Paul P Knegt 1, Cees A Meeuwis 4, Hemmo A Drexhage 5
PMCID: PMC11038436  PMID: 8299116

Abstract

A study was undertaken to help us reach a better understanding of the tumor-infiltrating pattern of lymphoid cells and in particular of monocyte-derived cells, namely the CD68+, acid-phosphatase-expressing scavenger macrophages and the MHC-class-II- and S100-antigen-presenting dendritic cells in head and neck squamous-cell carcinoma. In the stroma of the tumors distinctive small fields of lymphocytes were found, the T cell areas of these fields being intermingled with dendritic cells. Intra-epithelial dendritic cell infiltration was low. The infiltrative pattern of macrophages was similar to patterns described in earlier studies with substantial stromal invasion and inconsistent intra-epithelial invasion, but small granuloma-like structures of CD68+ macrophage-like cells, found in the stroma of tumors, have not been reported before. The histochemical localization of the tumor-infiltrated dendritic cells and macrophages supports the view that the former cells are involved in the sensitization to tumor antigens, whereas the latter cells are involved in tumor cytotoxicity/scavenging of tumor cell debris. Although it has been shown in the past that transmembranal (TM) factors (p15E-like factors) present in the serum and tumor of patients with cancer of the head and neck have suppressive effects on monocyte/macrophage/dendritic cell function, a relationship between the intensity of epithelial staining for TM factors and the infiltrative pattern of monocytes/macrophages/dendritic cells could not be demonstrated.

Key words: Macrophages, Dendritic cells, Head and neck cancer, Squamous-cell carcinoma

References

  • 1.Allen C, Hogg N. Elevation of infiltrating mononuclear phagocytes in human colorectal tumors. J Natl Cancer Inst. 1987;78:465. [PubMed] [Google Scholar]
  • 2.Austyn JM. Lymphoid dendritic cells. Immunology. 1987;62:161. [PMC free article] [PubMed] [Google Scholar]
  • 3.Balm AJM, Drexhage HA, von Blomberg M, Weltevreden EF, Veldhuizen RW, Mullink R, Snow GB. Mononuclear phagocyte function in head and neck cancer. Chemotactic responsiveness of blood monocytes in correlation between histologic grade of the tumor and infiltration of these cells into the tumor area. Cancer. 1984;54:1010. doi: 10.1002/1097-0142(19840915)54:6<1010::aid-cncr2820540613>3.0.co;2-n. [DOI] [PubMed] [Google Scholar]
  • 4.Cameron DJ, Stromberg BV. The ability of macrophages from head and neck cancer patients to kill tumor cells. Effect of prostaglandin inhibitors in cytotoxicity. Cancer. 1984;54:2403. doi: 10.1002/1097-0142(19841201)54:11<2403::aid-cncr2820541116>3.0.co;2-f. [DOI] [PubMed] [Google Scholar]
  • 5.Cianciolo GJ. Antiinflammatory proteins associated with human and murine neoplasms. Biochim Biophys Acta. 1986;865:69. doi: 10.1016/0304-419x(86)90014-4. [DOI] [PubMed] [Google Scholar]
  • 6.Cianciolo GJ, Herberman RB, Snyderman R. Depression of murine macrophage accululation by low molecular weight factors derived from spontaneous mammary carcinoma. J Natl Cancer Inst. 1980;65:829. doi: 10.1093/jnci/65.4.829. [DOI] [PubMed] [Google Scholar]
  • 7.van Dongen JJM, Adriaansen HJ, Hooijkaas H. Immunologic markers and their CD codes. J Immunol Methods. 1985;80:1. [Google Scholar]
  • 8.Garraud O, Faucher A, Legrand E. Impairment of monocyte functions in advanced head and neck cancer. Immunol Lett. 1988;18:213. doi: 10.1016/0165-2478(88)90021-1. [DOI] [PubMed] [Google Scholar]
  • 9.Guo M, Rabi BS, Johnson JT, Paradis IL (1987) Lymphocyte pheno-types at tumor margins in patients with head and neck cancer. Head Neck Surg? 265 [DOI] [PubMed]
  • 10.Gyure LA, Barfoot R, Denkan S, Hall JG. Immunity to a syngeneic sarcoma induced in rats by dendritic lymph cells exposed to tumor either in vivo or in vitro. Br J Cancer. 1987;55:17. doi: 10.1038/bjc.1987.4. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 11.Inoba K, Witmer MD, Steinman RM. Chistering of dendritic cells, helper T lymphocytes and histocompatible B cells during primary antibody responses in vitro. J Exp Med. 1984;160:858. doi: 10.1084/jem.160.3.858. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 12.Knight SC, Bedford P, Hunt R. The role of dendritic cells in the initiation of immune responses to contact sensitizers, IL studies in nude mice. Cell Immunol. 1985;94:435. doi: 10.1016/0008-8749(85)90267-9. [DOI] [PubMed] [Google Scholar]
  • 13.Milas L, Wike J, Hunter N, Volpe J, Basic I. Macrophage content of murine sarcomas and carcinomas: associations with tumor growth parameters and tumor radiocurability. Cancer Res. 1987;47:1069. [PubMed] [Google Scholar]
  • 14.Nakano T, Oka K, Arai T, Morita S, Tsunemoto H. Prognostic significance of Langerhans' cell infiltration in radiation therapy for squamous cell carcinoma of the uterine cervix. Arch Pathol Lab med. 1989;113:507. [PubMed] [Google Scholar]
  • 15.Neuchrist C, Grasl M, Scheiner O, Lassmann H, Ehrenberger K, Kraft D. Squamous cell carcinoma: infiltrating monocyte/macrophage subpopulations express functional mature phenotype. Br J Cancer. 1990;62:748. doi: 10.1038/bjc.1990.371. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 16.Nomori H, Watanabe S, Nakajima T, Shimosato Y, Kameya T. Histiocytes in nasopharyngeal carcinoma in relation to prognosis. Cancer. 1986;57:100. doi: 10.1002/1097-0142(19860101)57:1<100::aid-cncr2820570121>3.0.co;2-x. [DOI] [PubMed] [Google Scholar]
  • 17.Remels L, Fransen L, Huygen K, De Boetselier P. Immunological aspects of tumor-macrophage interactions. Adv Exp Med Biol. 1988;233:49. doi: 10.1007/978-1-4899-5037-6_7. [DOI] [PubMed] [Google Scholar]
  • 18.Tan IB, Drexhage HA, Mullink R, Hensen-Logmans S, De Haan-Meulman M, Snow GB, Balm AJM. Immunohistochemical detection of retroviral-p15E-related material in carcinomas of the head and neck. Otolaryngol Head Neck Surg. 1987;96:251. doi: 10.1177/019459988709600304. [DOI] [PubMed] [Google Scholar]
  • 19.Tas MPR, Simons PJ, Balm FJM, Drexhage HA. Depressed monocyte polarization and clustering of dendritic cells in patients with head and neck cancer: in vitro restoration of this immunosuppression by thymic hormones. Cancer Immunol Immunother. 1993;36:108. doi: 10.1007/BF01754410. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 20.Tsujitani S, Furukawa T, Tamada R, Okamura T, Yasumoto K, Sugimachi K. Langerhans cells and prognosis in patients with gastric carcinoma. Cancer. 1987;59:501. doi: 10.1002/1097-0142(19870201)59:3<501::aid-cncr2820590325>3.0.co;2-h. [DOI] [PubMed] [Google Scholar]
  • 21.Tsujitani S, Kakeji Y, Watanabe A, Kohnoe S, Maehara Y, Sugimachi K. Infiltration of dendritic cells in relation to tumor invasion and lymph node metastasis in human gastric cancer. Cancer. 1990;66:2012. doi: 10.1002/1097-0142(19901101)66:9<2012::aid-cncr2820660928>3.0.co;2-n. [DOI] [PubMed] [Google Scholar]
  • 22.Wolf GT, Hudson JL, Peterson KA, Miller HL, McLatchey KD. Otolaryngol Head Neck Surg. 1986;95:142. doi: 10.1177/019459988609500203. [DOI] [PubMed] [Google Scholar]
  • 23.Yoshida K, Tachibana T. Prevention of lymph node metastases by adoptive transfer of CD4+ T lymphocytes admixed with irradiated tumor cells. Cancer Immunol Immunother. 1993;36:323. doi: 10.1007/BF01741171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 24.Zuk JA, Walker RA. Immunohistochemical analysis of HLA antigens and mononuclear infiltrates of benign and malignant breast. J Pathol. 1987;152:275. doi: 10.1002/path.1711520406. [DOI] [PubMed] [Google Scholar]

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