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. 1994 May;39(3):161–166. doi: 10.1007/BF01533381

Increased proliferation of a human breast carcinoma cell line by recombinant interleukin-2

Mitsuo Katano 1,✉, Tatsuya Matsuo 1, Takashi Morisaki 2, Keiko Naito 3, Fumio Nagumo 4, Eiro Kubota 5, Mitsunari Nakamura 1, Takeharu Hisatsugu 1, Jutaro Tadano 4
PMCID: PMC11038457  PMID: 7923245

Abstract

Two adenocarcinoma cell lines, Breast M25-SF and Breast M, were established from tumor tissue resected surgically from a patient with breast cancer. One, Breast M25-SF, expresses interleukin-2 receptor (IL-2R) on the cell surface and the other, Breast M, does not. The effects of recombinant inteleukin-2 (rIL-2) on the proliferation of these cell lines were investigated. The growth of Breast M25-SF was significantly promoted by rIL-2 ranging from 1,25 U/ml to 640 U/ml. Anti-CD25 (Tac) antibody, significantly blocked the growth enhancement of Breast M25-SF by rIL-2. Breast M, however, did not respond to rIL-2. To confirm more directly the promotion of Breast M25-SF growth by rIL-2, cloning of IL-2 responders from parent Breast M25-SF cells was carried out by limiting dilution without feeder cells in 96-well microplates. No colony formation was found in 24 wells without rIL-2. Eleven, 13 and 6 clones were established from groups of 24 wells containing rIL-2 at 200, 20 and 2 U/ml respectively. All of the clones expressed IL-2R and respond to rIL-2. By using a sensitive polymerase chain reaction technique, we demonstrated that Breast M25-SF but not Breast M expressed IL-2 mRNA, and IL-2 secretion from Breast M25-SF but not Breast M was also confirmed by radioimmunoassay. These findings suggest a role for IL-2 in autocrine support of Breast M25-SF growth. IL-2 may play an important role in the growth control of breast carcinoma cells.

Key words: Breast carcinoma cell line, Interleukin-2, Interleukin-2 receptor, Autocrine growth factor, Interleukin-2 secretion

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