Abstract
A selection of 16 monoclonal antibodies has been produced against a fresh Ewing's sarcoma (ES) tumor mixed with a permanent ES cell line. The majority of antibodies identify an 80-kDa molecule, which is not detected on healthy tissues except on certain cultured monocytes. One antibody recognize the CD2 ligand MIC2 and 2 antibodies (numbers 13 and 16) define a higher-molecular-mass antigen. Antibody 16 is also expressed on mesenchymal fibroblasts of bone marrow or fetal origin. Tumorspecific antigen expression is potentially linked to the chromosome 22 abnormality decribed in Ewing's sarcoma.
Key words: Ewing's sarcoma, Tumor-specific monoclonal antibodies, MIC-2
References
- 1.Aurias A, Rimbaut C, Buffe D, Dubou J, Mazabraud A. Chromosomal translocations in Ewing's sarcoma. N Engl J Med. 1983;309:496. doi: 10.1056/NEJM198308253090817. [DOI] [PubMed] [Google Scholar]
- 2.Brown TJ, Lioubin MN, Marquardt H. Purification and characterization of cytostatic lymphokines produced by activated human T lymphocytes. J Immunol. 1987;139:2977. doi: 10.4049/jimmunol.139.9.2977. [DOI] [PubMed] [Google Scholar]
- 3.Delattre O, Zucman J, Plougastel B, Desmaze C, Melot T, Peter M, Kovar H, Joubert I, de Jong P, Rouleau G, Aurias A, Thomas G. Gene fusion with an ETS DNA-binding domain caused by chromosome translocation in human tumours. Nature. 1992;359:162. doi: 10.1038/359162a0. [DOI] [PubMed] [Google Scholar]
- 4.Donnet LR. Cytogenetics and molecular biology of small round cell tumors and related, neoplasms: current status. Cancer Genet Cytogenet. 1991;54:1. doi: 10.1016/0165-4608(91)90023-N. [DOI] [PubMed] [Google Scholar]
- 5.Fellinger EJ, Garin-Chesa P, Su SL, DeAngelis P, Lane JM, Rettig WJ. Biochemical and genetic characteization of the HBA71 Ewing's sarcoma cell surface antigen. Cancer Res. 1991;51:336. [PubMed] [Google Scholar]
- 6.Gelin C, Aubrit F, Phalipon A, Raynal B, Cole S, Kaczorek M, Bernard A. The E2 antigen, a 32 kd glycoprotein involved in T-cell adhesion processes, is the MIC2 gene product. EMBO J. 1989;8:3253. doi: 10.1002/j.1460-2075.1989.tb08485.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 7.Guarini L, Temponi M, Bruce JN, Bollon AP, Duigou GJ, Moulton TA, Ferrone S, Fisher PB. Expression and modulation by cytokines of the intercellular adhesion molecule-1 (ICAM-1) in human central nervous system tumor cell cultures. Int J Cancer. 1990;46:1041. doi: 10.1002/ijc.2910460616. [DOI] [PubMed] [Google Scholar]
- 8.Hamilton G, Mallinger R, Havel M. Ewing's-sarcoma-associated HBA-71 tumor antigen represents a new differentiation marker of human thymocytes. J Cancer Res Clin Oncol. 1989;115:592. doi: 10.1007/BF00391364. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 9.Hara S, Ishii E, Tanaka S, Yokoyama J, Katsumata K, Fujimoto J, Hata J. A monoclonal antibody specifically reactive with Ewing's sarcoma. Br J Cancer. 1989;60:873. doi: 10.1038/bjc.1989.383. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 10.Jeffery E, Prive V, Gearing DP. Close preximity of the genes for leukemia inhibitory factor and oncostatin M. Cytokine. 1993;5:107. doi: 10.1016/1043-4666(93)90048-A. [DOI] [PubMed] [Google Scholar]
- 11.Rodriguez E, Sreekantaiah C, Gerald W, Reuter VE, Motzer RJ, Chaganti RSK. A recurring translocation t(11;22) (p13;q11. 2), characterizes intra-abdominal desmoplastic small round-cell tumors. Cancer Gent Cytogenet. 1993;69:17. doi: 10.1016/0165-4608(93)90105-U. [DOI] [PubMed] [Google Scholar]
- 12.Sainati L, Stella M, Montaldi A, Bolcato S, Guercini N, Bonan F, Ninfo V, Zanesco L, Basso G. Value of cytogenetics in the differential diagnosis of small round cell tumors in childhood. Med Pediatr Oncot. 1992;20:130. doi: 10.1002/mpo.2950200208. [DOI] [PubMed] [Google Scholar]
- 13.Turc-Carel C, Philips I, Berger MP, Philip T, Lenoir GM. Chromosomal translocation in Ewing's sarcoma. J Engl J Med. 1983;309:497. [PubMed] [Google Scholar]
- 14.Whang-Peng J, Triche TJ, Knutsen T, Miser J, Kao-Shan S, Tsai S, Israel MA. Cytogenetic characterization of selected small round cell tumors of childhood. Cancer Genet Cytogenet. 1986;21:185. doi: 10.1016/0165-4608(86)90001-4. [DOI] [PubMed] [Google Scholar]
- 15.Zucman J, Delattre O, Desmaze C, Plougastel B, Joubert I, Melot T, Peter M, De Jong P, Rouleau G, Aurias A, Thomas G. Cloning and characterization of the Ewing's sarcma and peripheral neuroepithelioma t(11;22) translocation and breakpoints. Genes Chromosomes Cancer. 1992;5:271. doi: 10.1002/gcc.2870050402. [DOI] [PubMed] [Google Scholar]