Abstract
The uptake of a monoclonal antibody (OX7) by murine lymphomas (SL1, SL2) growing in two sites in the mouse were compared. SL2 tumors grown in the subrenal site showed greater specific antibody uptake than did the same tumor grown in the subcutaneous site. Major differences in membrane bound antibody, in vitro antibody binding patterns, and gamma scintillation camera imaging were also observed between the two sites. These differences may be due to the greater blood flow measured in tumors growing in the subrenal capsule than those growing at the subcutaneous site. The differences observed in antibody uptake of the same tumor growing in two different sites raises questions concerning the choice of animal model systems that can be used to predict clinical utility.
Keywords: Lymphoma, Monoclonal Antibody, Clinical Utility, Antibody Binding, Camera Imaging
References
- 1.Accolla RS, Carrel S, Mach J-P. Monoclonal antibodies specific for carcinoembroyonic antigen and produced by two hybrid cell lines. Proc Natl Acad Sci USA. 1980;77:563–566. doi: 10.1073/pnas.77.1.563. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 2.Ackerman NB, Hechmer PA. Comparison of vascular permeability of tumors and liver host tissues. Bibl Anat. 1977;15:304–306. [PubMed] [Google Scholar]
- 3.Ballou B, Levine G, Hakala RJ, Solter D. Tumor location detected with radioactively labelled monoclonal antibodies and external scintigraphy. Science. 1979;206:844–847. doi: 10.1126/science.493985. [DOI] [PubMed] [Google Scholar]
- 4.Bogden A, Kelton D, Cobb W, Esber H (1978) A rapid screening method for testing chemotherapeutic agents against human xenografts, In: Houchens DP, Ovejera AA, Fischer Gustav (eds) Proceedings of Symposium on the Use of Athymic (Nude) Mice in Cancer Research. New York, pp 231–250
- 5.Colcher D, Keenan AM, Larson SM, Schlom J. Prolonged binding of a radiolabeled monoclonal antibody (B72.3) used in the in situ radioimmunodetection of human colon carcinoma xenografts. Cancer Res. 1981;44:5744–5751. [PubMed] [Google Scholar]
- 6.Colcher D, Zalutsky M, Kaplan W, Kufe D, Austin F, Schlom J. Radiolocalization of human mammary tumors in athymic mice by a monoclonal antibody. Cancer Res. 1983;43:736–742. [PubMed] [Google Scholar]
- 7.Gallagher BM. Monoclonal antibodies. The design of appropriate cancer and evaluation systems. In: Lambrecht R, Eckelman WC, editors. Animal Models in Radiotracer Design. New York: Springer-Verlag; 1983. pp. 66–105. [Google Scholar]
- 8.Gullino PM, Grantham FH. Studies on the exchange of fluids between host and tumor. II. The blood flow of hepatomas and other tumors in rats and mice. J Natl Cancer Inst. 1961;27:1465–1491. [PubMed] [Google Scholar]
- 9.Haskell CM, Buchegger F, Schreyer M, Carrel S, Mach J-P. Monoclonal antibodies to carcinoembryomic antigen: ionic strength as a factor in the selection of antibodies for immunoscintography. Cancer Res. 1983;43:3857–3864. [PubMed] [Google Scholar]
- 10.Houston LL, Nowinski RC, Bernstein ID. Specific localization of monoclonal antibodies directed against the Thy 1.1 antigen. J Immunol. 1980;125:837–843. [PubMed] [Google Scholar]
- 11.Khazaeli MB, Grown LE, Kabaza GA, Sargent EW, Beierwaltes WH. Selection of monoclonal antibodies to HCG which localizes in human choriocarcinoma growing in the Syrian hamster cheek pouch. Hybridoma. 1984;3:41–48. doi: 10.1089/hyb.1984.3.41. [DOI] [PubMed] [Google Scholar]
- 12.Kohler G, Milstein C. Continuous culture of fused cell secreting antibody of predefined specificity. Nature. 1975;256:495–497. doi: 10.1038/256495a0. [DOI] [PubMed] [Google Scholar]
- 13.Midoux P, Maillet T, Therain F, Monsigny M, Roche AC. Tumor localization of Lewis lung carcinoma with radiolabeled monoclonal antibodies. Cancer Immunol Immunother. 1984;18:19–23. doi: 10.1007/BF00205394. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 14.Moldofsky PJ, Powe J, Mulhern CB, Hammond N, Sears HF, Gatenby RA, Steplewski Z, Koprowski H. Metastatic colon carcinoma, detected with radiolabeled F(ab')2 monoclonal antibody fragments. Radiology. 1983;149:549–555. doi: 10.1148/radiology.149.2.6622704. [DOI] [PubMed] [Google Scholar]
- 15.Moshakis V, McIlhinney RAJ, Raghavan D, Neville AM. Localization of human tumour xenografts after I. V. administration of radiolabelled monoclonal antibodies. Br J Cancer. 1981;44:91–99. doi: 10.1038/bjc.1981.153. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 16.Sands H, Gallagher BM, Jones PL, Vaccaro D, Camin LL. Evaluation of the “Thy System” for exploring the potential of monoclonal antibodies for tumor imaging and drug delivery. Int J Immunopharmacol. 1982;4:29. [Google Scholar]
- 17.Sands H, Jones PJ, Neacy W, Shah SA, Gallagher BM. The imaging of small experimental murine tumors grown in the subrenal capsule using monoclonal antibodies. Cancer Lett. 1984;24:65–72. doi: 10.1016/0304-3835(84)90081-8. [DOI] [PubMed] [Google Scholar]
- 18.Sands H, Shah SA, Gallagher BM. Vascular volume and permeability of human and murine tumors grown in Athymic mice. Cancer Lett. 1985;27:15–21. doi: 10.1016/0304-3835(85)90004-7. [DOI] [PubMed] [Google Scholar]
- 19.Scheinberg DA, Strand M, Gansow OA. Tumor imaging with radioactive metal chelates conjugated to monoclonal antibodies. Science. 1982;215:1511–1513. doi: 10.1126/science.7199757. [DOI] [PubMed] [Google Scholar]
- 20.Wahl RL, Parker CW, Philpott GW. Improved radioimaging and tumor localization with monoclonal F(ab')2 . J Nucl Med. 1983;24:316–325. [PubMed] [Google Scholar]