Abstract
The anticancer drugs Adriamycin, bleomycin, vincristine, 4-hydroperoxycyclophosphamide, FK156 and FK565 and the immunosuppressive drug cyclosporin-A were tested for their effect on lipopolysaccharide-induced tumour necrosis factor-α (TNF) release from rat peritoneal exudate cells in vitro. FK565 resulted in a marked enhancement of TNF release when added to cultures at doses of 1 µg/ml and 10 µg/ml. However, FK156, Adriamycin, bleomycin, 4-hydroperoxycyclophosphamide, vincristine and cyclosporin A did not affect the release of TNF under the same conditions. Puromycin inhibited the release of TNF in the same system at non-cytotoxic doses of 1 µg/ml and 10 µg/ml.
Keywords: Cancer Research, Tumour Necrosis Factor, Cyclosporin, Anticancer Drug, Vincristine
References
- 1.Abdul Hamied TA, Turk JL. Enhancement of interleukin-2 release in rats by treatment with bleomycin and adriamycin in vivo. Cancer Immunol Immunother. 1987;25:245. doi: 10.1007/BF00199154. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 2.Abdul Hamied TA, Parker D, Turk JL. Potentiation of release of interleukin-2 by bleomycin. Immunopharmacology. 1986;12:27. doi: 10.1016/0162-3109(86)90038-x. [DOI] [PubMed] [Google Scholar]
- 3.Abdul Hamied TA, Parker D, Turk JL. Effects of Adriamycin, 4-hydroperoxy cyclophosphamide and ASTA-Z (INN Mafosfamide) on the release of IL-2 and IL-1 in vitro. Int J Immunopharmacol. 1987;9:35. doi: 10.1016/0192-0561(87)90061-0. [DOI] [PubMed] [Google Scholar]
- 4.Ahmed K, Turk JL. Effect of anticancer agents neothramycin, adacinomycin, FK565 and FK156 on the release of interleukin-2 and interleukin-1 in vitro. Cancer Immunol Immunother. 1989;28:87–92. doi: 10.1007/BF00199107. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 5.Ahmed K, Abdul Hamied TA, Turk JL. Inhibition of release of interleukin-2 by vincristine and vinblastine. Immunopharmacol Immunotoxicol. 1987;9:391. doi: 10.3109/08923978709035221. [DOI] [PubMed] [Google Scholar]
- 6.Bate CAW, Taverne J, Playfair JHL. Soluble malarial antigens are toxic and induce the production of tumour necrosis factor in vivo. Immunol. 1989;66:600. [PMC free article] [PubMed] [Google Scholar]
- 7.Beutler B, Mahoney J, Le Trang N, Pakela P, Cerami A. Purification of cachectin, a lipoprotein lipase suppressing hormone secreted by endotoxin induced RAW 264.7 cells. J Exp Med. 1985;161:984. doi: 10.1084/jem.161.5.984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 8.Bunjes D, Hardt C, Rollinghoff M, Wagner H. Cyclosporin-A mediates immunosuppression of primary cytotoxic T cell responses by impairing release of interleukin-1 and interleukin-2. Eur J Immunol. 1981;11:657. doi: 10.1002/eji.1830110812. [DOI] [PubMed] [Google Scholar]
- 9.Currie GA, Basham C. Activated macrophages release a factor which lyses malignant fells but not normal cells. J Exp Med. 1985;142:1600. doi: 10.1084/jem.142.6.1600. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 10.Dammaco F, Vacca A, Benevestito S, Pantales G. Tumour necrosis factor; biologic and immunochemical properties and potential therapeutic applications. Recent advances in autoimmunity and tumour immunology. Milano: ed Ermes; 1988. pp. 157–168. [Google Scholar]
- 11.Dinarello CA. Interleukin-1. Rev Infect Dis. 1984;6:51. doi: 10.1093/clinids/6.1.51. [DOI] [PubMed] [Google Scholar]
- 12.Hotterman OA, Klein E, Casale GP. Selective cytotoxicity peritoneal leucocytes for neoplastic cells. Cell Immunol. 1973;9:339. doi: 10.1016/0008-8749(73)90049-x. [DOI] [PubMed] [Google Scholar]
- 13.Izumi S, Nakahera K, Gotoh T, Hashimoto S, Kino T, Okuhara M, Aoki H, Inanaka H. Antitumour effects of novel immunoactive peptide FK156 and its synthetic derivatives. J Antibiot (Tokyo) 1983;36:566. doi: 10.7164/antibiotics.36.566. [DOI] [PubMed] [Google Scholar]
- 14.Meyers FH, Jawetz E, Goldfien A. Review of medical pharmacology. 5th ed. California: Lange; 1976. p. 577. [Google Scholar]
- 15.Mine Y, Watanabe Y, Tawara S, Yokoto Y, Nishida M. Immunoactive peptides FK156, FK565 — enhancement of host defence mechanisms against infections. J Antibiot (Tokyo) 1983;36:1045. doi: 10.7164/antibiotics.36.1059. [DOI] [PubMed] [Google Scholar]
- 16.Thomson AW, Moon DK, Nelson DS. Suppression of delayed type hypersensitivity reactions and lymphokine production by cyclosporin-A in the mouse. Clin Exp Immunol. 1983;52:599. [PMC free article] [PubMed] [Google Scholar]
- 17.Watanabe Y, Tawara S, Mine Y, Kikuchi H. Immunoactive peptides FK156 and FK565 — activation of mouse macrophages. J Antibiot (Tokyo) 1985;38:1781. doi: 10.7164/antibiotics.38.1781. [DOI] [PubMed] [Google Scholar]