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. 1992 Jul;35(4):230–236. doi: 10.1007/BF01789328

Detection of allogeneic Qa/TL and Ly specificities on murine tumor cells with IgD in tumor-regressor serum

Takae Tanino 1, Naohiro Seo 1, Takahiro Okazaki 1, Chiemi Nakanishi-Ito 1, Masayuki Sekimata 1, Kohji Egawa 1,✉
PMCID: PMC11038994  PMID: 1380893

Abstract

Serum from C3H/He mice, which show regression of MM2 tumor cells after transplantation and removal (regressor serum, RS) contains non-gammaglobulin components that cross-react with various tumor cells of mice [22, 23]. In addition to tumor cells, various allogeneic lymphocytes are also susceptible to an RS-dependent lymphocyte-mediated cytotoxic reaction. To identify tumor cell surface antigens that cause the cross-reactive host response, the serum components were analyzed by absorption of RS with allogeneic lymphocytes. RS components were found to recognize allogeneic lymphocyte antigens including Qa-2 and Ly6.2. Specificity for the Qa-2 antigen was further tested using Qa-2-congenic mice. The expression of Qa-2 antigen was detected on the surfaces of MM2 and other tumor cells derived from H-2k mice (seven among nine cell lines tested) by a membrane immunofluorescence method using a Qa-2-specific mAb. Physical characteristics of the Qa-2-specific component in RS were determined and found to differ from those of regular IgGs but to be similar to those of IgDs. Using an enzyme-linked immunosorbent assay with an IgD-specific mAb and Qa-2-lacZ fusion protein, the existence of IgD in RS with specificity for Qa-2 was confirmed. These results suggest that the RS component with Qa-2 specificity is an IgD, the specificity and physiological role of which are unknown.

Key words: Tumor antigen, Qa-2, IgD

References

  • 1.Boyle W. An extension of the51Cr-release assay for the estimation of cytotoxins. Transplantation. 1968;6:761. doi: 10.1097/00007890-196809000-00002. [DOI] [PubMed] [Google Scholar]
  • 2.Boyse EA, Old LJ, Chouroulinkov I. Cytotoxicity test for demonstration of mouse antibody. Methods Med Res. 1964;10:39. [PubMed] [Google Scholar]
  • 3.Davis BJ. Disc electrophoresis: II. Method and application to human serum proteins. Ann NY Acad Sci. 1964;121:404. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
  • 4.Finkelman FD, Kessler SW, Mushinski JF, Potter M. IgD-secreting murine plasmacytomas: identification and partial characterization of two IgD myeloma proteins. J Immunol. 1981;126:680. [PubMed] [Google Scholar]
  • 5.Greenwood HC, Hunter WM, Glover JS. The preparation of131I-labelled human growth hormone of high specific radioactivity. Biochem J. 1963;89:114. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 6.Ishihara E, Tejima Y, Takahashi R, Takayasu T, Shinoda T. Structure and location of asparagine-linked olygosaccharides in the Fc region of a human immunoglobulin D. Biochem Biophys Res Commun. 1983;110:181. doi: 10.1016/0006-291x(83)91277-9. [DOI] [PubMed] [Google Scholar]
  • 7.Julius MH, Simpson E, Herzenberg LA. A rapid method for isolation of functional thymus-derived murine lymphocytes. Eur J Immunol. 1973;3:645. doi: 10.1002/eji.1830031011. [DOI] [PubMed] [Google Scholar]
  • 8.Kimura Y, Tanino-Komaiji T. Survival of host mice and induced resistance to transplantable ascites tumors. Jpn J Exp Med. 1966;36:371. [PubMed] [Google Scholar]
  • 9.Klein J, Figueroa F, David CS. H-2 haplotypes, gene and antigens: second listing. Immunogenetics. 1983;17:553. doi: 10.1007/BF00366126. [DOI] [PubMed] [Google Scholar]
  • 10.McKenzie IFC, Potter T. Murine lymphocyte surface antigens. Adv Immunol. 1979;27:179. doi: 10.1016/s0065-2776(08)60263-1. [DOI] [PubMed] [Google Scholar]
  • 11.Mellor AL, Antoniou J, Robinson PJ. Structure and expression of genes encoding Qa-2 class I gene. Proc Natl Acad Sci USA. 1985;82:5920. doi: 10.1073/pnas.82.17.5920. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 12.Mountz JD, Mushinski JF, Owens JD, Finkelman FD. The in vivo generation of murine IgD-secreting cells is accompanied by deletion of Cµ gene and occasional deletion of the gene for the Cδ1 domain. J Immunol. 1990;145:1583. [PubMed] [Google Scholar]
  • 13.O'Farrell PZ, Goodman HM, O'Farrell PH. High resolution two-dimensional electrophoresis of basic as well as acidic proteins. Cell. 1977;12:1133. doi: 10.1016/0092-8674(77)90176-3. [DOI] [PubMed] [Google Scholar]
  • 14.Rigby PW, Brickell PM, Latchman DS, Murphy D, Scott MRD, Westphal KH, Simains V, Lane DP, Willison K. Cancer cells: transformed phenotype. New York: Cold Spring Harbor Laboratory; 1984. Oncofetal genes and major histocompatibility complex; p. 97. [Google Scholar]
  • 15.Seo N, Okazaki T, Nakanishi-Ito C, Tanino T, Matsudaira Y, Takahashi T, Egawa K. Expression of the Qa-2k phenotype encoded by the Q5k gene on the surface of tumor cells derived from H-2k mice. J Exp Med. 1992;175:647. doi: 10.1084/jem.175.3.647. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 16.Sharrow SO, Arn JS, Stroynowski I, Hood L, Sachs DH. Epitope clusters of Qa-2 antigens defined by a panel of new monoclonal antibodies. J Immunol. 1989;142:3495. [PubMed] [Google Scholar]
  • 17.Shinoda T. Primary structures of immunoglobulins. In: Onoe K, Utsumi S, editors. Immunoglobulins. Tokyo: Iwanami Shoten; 1983. p. 15. [Google Scholar]
  • 18.Soloski MJ, Hood L, Stroynovski I. Qa-region class I gene expression: Identification of a second class I gene, Q9, encoding a Qa-2 polypeptide. Proc Natl Acad Sci USA. 1988;85:3100. doi: 10.1073/pnas.85.9.3100. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 19.Spiegelberg HL. The structure and biology of human IgD. Immunol Rev. 1977;37:3. doi: 10.1111/j.1600-065x.1977.tb00243.x. [DOI] [PubMed] [Google Scholar]
  • 20.Spiegelberg HL. Immunoglobulin D (IgD) Methods Enzymol. 1985;116:95. doi: 10.1016/s0076-6879(85)16007-6. [DOI] [PubMed] [Google Scholar]
  • 21.Stanton TH, Boyse EA. A new serologically defined locus, Qa-1, in the Tla-region of the mouse. Immunogenetics. 1976;3:525. [Google Scholar]
  • 22.Tanino T. Studies on tumor-host relationship: II. Serum resistance factor(s) produced by tumor transplantation. Jpn J Exp Med. 1968;38:383. [PubMed] [Google Scholar]
  • 23.Tanino T, Egawa K. Regressor serum factor-dependent nonspecific killers in tumor-bearing mice. Jpn J Exp Med. 1986;56:163. [PubMed] [Google Scholar]
  • 24.Tanino T, Saito M, Egawa K. Cell line-specific cell surface component of hypotetraploid ascites mammary carcinoma cells inducing humoral reactions in the syngeneic host. Int J Cancer. 1976;17:736. doi: 10.1002/ijc.2910170608. [DOI] [PubMed] [Google Scholar]
  • 25.Tomono T, Ikeda H, Tokunaga E. High-performance ion-exchange chromatography of plasma proteins. J Chromatorgr. 1983;266:39. doi: 10.1016/s0021-9673(01)90877-5. [DOI] [PubMed] [Google Scholar]
  • 26.Watts S, Davis AC, Gaut B, Wheeler C, Hill L, Goodenow RS. Organization and structure of the Qa genes of the major histocompatibility complex of the C3H mouse: implications for Qa function and class I evolution. EMBO J. 1989;8:1749. doi: 10.1002/j.1460-2075.1989.tb03568.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 27.Weiss EH, Golden L, Fahrner K, Mellor AL, Devlin JJ, Bullman H, Tiddens H, Bud H, Flavell RA. Organization and evolution of the class I gene family in the major histocompatibility complex of the C57BL/10 mouse. Nature. 1984;310:650. doi: 10.1038/310650a0. [DOI] [PubMed] [Google Scholar]
  • 28.Zarling JM, Robins HI, Raich PC, Bach FH, Bach ML. Generation of cytotoxic T lymphocytes to autologous human leukemia cells by sensitisation to pooled allogeneic normal cells. Nature. 1978;274:269. doi: 10.1038/274269a0. [DOI] [PubMed] [Google Scholar]

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