Skip to main content
NCBI home page
Cancer Immunology, Immunotherapy : CII logoLink to Cancer Immunology, Immunotherapy : CII
. 1985 Feb;19(1):62–67. doi: 10.1007/BF00199314

Further studies on the differences in cytotoxicity of human peripheral blood monocytes and bronchoalveolar macrophages for cultured human lung cells

Stuart Swinburne 1,✉, Michael Moore 3, Peter Cole 3
PMCID: PMC11039032  PMID: 3844975

Abstract

Previously reported differences between the cytostatic activity of human peripheral blood monocytes (PBM) and bronchoalveolar macrophages (BAM) for cultured human lung tumor cells have been further investigated. The differences are both quantitative and qualitative and are shown not to be due to the respective methods of purification. There was a varying contribution of cytolysis to the cytostasis detected by the 75selenomethionine post-labeling assay used. Bronchoalveolar macrophages were cytolytic when tested at both low and high E: T ratios but PBM were only cytolytic at the low E: T ratio. A variable dependence upon soluble cytostatic factor(s) was suggested, and there was evidence of heterogeneity in the factors released by the two populations. Cytostatic factor production by both populations appeared to be under similar regulatory constraints. In vitro maturation of PBM altered their cytostatic dose-response curve to one resembling that previously reported for BAM. It was also shown that sera from poor-prognosis lung tumor patients, which suppressed the in vitro maturation of PBM, also suppressed the in vitro cytostatic activity of PBM for cultured human lung tumor cells.

Keywords: Cancer Research, Human Lung, Lung Tumor, Factor Production, Tumor Patient

References

  • 1.Balkwell FR, Hogg N. Characterisation of human breast milk macrophages cytostatic for human cell lines. J Immunol. 1979;123:1451. [PubMed] [Google Scholar]
  • 2.Binderup L, Bramm E, Arrigone-Martelli E. Peritoneal macrophages from adjuvant arthritis rats enhance tumor cell growth in vitro. Experientia. 1978;35:1230. doi: 10.1007/BF01963307. [DOI] [PubMed] [Google Scholar]
  • 3.Blusse van oud Alblas A, van Furth R. Origin, kinetics and characteristics of pulmonary macrophages in the normal steady state. J Exp Med. 1979;149:1504. doi: 10.1084/jem.149.6.1504. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 4.Cabilly S, Gallily R. Studies on the recognition of xenogeneic cells by non-immune macrophages. I. Destruction of chicken fibroblasts in vitro by murine macrophages. Cell Immunol. 1977;29:54. doi: 10.1016/0008-8749(77)90274-x. [DOI] [PubMed] [Google Scholar]
  • 5.Cameron DJ, Hallowell Churchill W. Cytotoxicity of human macrophages for tumor cells: enhancement by human lymphocyte mediators. J Clin Invest. 1979;63:977. doi: 10.1172/JCI109398. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 6.Cameron DJ, Hallowell Churchill W. Cytotoxicity of human macrophages for tumor cells: enhancement by bacterial lipopolysaccharides (LPS) J Immunol. 1980;124:708. [PubMed] [Google Scholar]
  • 7.Coggle JE, Tarling JD. Cell kinetics of pulmonary alveolar macrophages in the mouse. Cell Tissue Kinet. 1982;15:139. doi: 10.1111/j.1365-2184.1982.tb01032.x. [DOI] [PubMed] [Google Scholar]
  • 8.Cohen AB, Cline MJ. In vitro studies of the foamy macrophage of postobstructive endogenous lipoid pneumonia in man. Am Rev Respir Dis. 1972;106:69. doi: 10.1164/arrd.1972.106.1.69. [DOI] [PubMed] [Google Scholar]
  • 9.DeLustro F, Sherer GK, LeRoy EC. Human monocyte stimulation of fibroblast growth by a soluble mediator(s) J Reticuloendothel Soc. 1980;28:519. [PubMed] [Google Scholar]
  • 10.Dent R, Cole P. In vitro monocyte maturation as a prediction of survival in squamous cell carcinoma of the lung. Thorax. 1981;36:446. doi: 10.1136/thx.36.6.446. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 11.Dent RG, Cole PJ. In vitro monocyte maturation in squamous-cell carcinoma of the lung: influence of humoral factors. Br J Cancer. 1982;46:522. doi: 10.1038/bjc.1982.88. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 12.Fray A, Lorinet AM, Thobie N, Halpern B. Equal sensitivity of normal or tumoural fibroblasts to cytotoxic and cytostatic effects of normal or Corynebacterium parvum-activated peritoneal exudate cells. Ann Immunol [C] 1979;130:445. [PubMed] [Google Scholar]
  • 13.Goldman R, Bar-Shavit Z. Dual effect of normal and stimulated macrophages and their conditioned media on target cell proliferation. J Natl Cancer Inst. 1979;63:1009. [PubMed] [Google Scholar]
  • 14.Goldman R, Hogg N. Enhanced susceptibility of virus-infected fibroblasts to cytostasis mediated by peritoneal exudate cells. J Immunol. 1978;121:1657. [PubMed] [Google Scholar]
  • 15.Hammerstrom J. Human macrophage differentiation in vivo and in vitro. A comparison of human peritoneal macrophages and monocytes. Acta Pathol Microbiol Scand [C] 1979;87:113. [PubMed] [Google Scholar]
  • 16.Hammerstrom J. Human adherent mononuclear blood cells; cytolytic and cytostatic activity and characterisation of effector cells during in vitro culture. Acta Pathol Microbiol Scand [C] 1981;88:201. doi: 10.1111/j.1699-0463.1981.tb02674.x. [DOI] [PubMed] [Google Scholar]
  • 17.Johnson KJ, Ward PA, Striker G, Kunkel R. A study of the origin of pulmonary macrophages using the Chediak-Higashi marker. Am J Pathol. 1980;101:365. [PMC free article] [PubMed] [Google Scholar]
  • 18.Lawson ADG, Stevenson GT. Macrophages induce antibody-dependent cytostasis but not lysis in guinea pig leukaemic cells. Br J Cancer. 1983;48:227. doi: 10.1038/bjc.1983.178. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 19.Lemarbre P, Hoidal J, Vessella R, Rinehart J. Human pulmonary macrophage tumor cell cytotoxicity. Blood. 1980;55:612. [PubMed] [Google Scholar]
  • 20.Liebovich SJ, Ross R. A macrophage-dependent factor that stimulates the proliferation of fibroblasts in vitro. Am J Pathol. 1976;84:501. [PMC free article] [PubMed] [Google Scholar]
  • 21.Mannel DN, Falk W, Meltzer MS. Inhibition of nonspecific tumoricidal activity by activated macrophages with antiserum against a soluble cytotoxic factor. Infect Immun. 1981;33:156. doi: 10.1128/iai.33.1.156-164.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 22.Mantovani A. In vitro effects on tumor cells of macrophages isolated from an early-passage chemically induced murine sarcoma and from its spontaneous metastases. Int J Cancer. 1981;27:221. doi: 10.1002/ijc.2910270215. [DOI] [PubMed] [Google Scholar]
  • 23.Mantovani A, Jerrells TR, Dean JH, Herberman RH. Cytolytic and cytostatic activity on tumor cells of circulating human monocytes. Int J Cancer. 1979;23:18. doi: 10.1002/ijc.2910230105. [DOI] [PubMed] [Google Scholar]
  • 24.Mantovani A, Tagliabue A, Dean JH, Jerrells TR, Herberman RB. Cytolytic activity of circulating human monocytes on transformed and untransformed human fibroblasts. Int J Cancer. 1979;23:28. doi: 10.1002/ijc.2910230106. [DOI] [PubMed] [Google Scholar]
  • 25.Matthews N. Production of an anti-tumour cytotoxin by human monocytes. Immunology. 1981;44:135. [PMC free article] [PubMed] [Google Scholar]
  • 26.Matthews N. Effect on human monocyte killing of tumour cells of antibody raised against an extracellular monocyte cytotoxin. Immunology. 1983;48:321. [PMC free article] [PubMed] [Google Scholar]
  • 27.Pertoft H, Johnsson A, Warmegard B, Seljelid R. Separation of human monocytes on density gradients of Percoll. J Immunol Methods. 1980;33:221. doi: 10.1016/0022-1759(80)90209-4. [DOI] [PubMed] [Google Scholar]
  • 28.Reidarson TH, Levy WE, Klostergaard J, Granger GA. Inducible macrophage cytotoxins. I. Biokinetics of activation and release in vitro. JNCI. 1982;69:867. [PubMed] [Google Scholar]
  • 29.Rinehart J, Lange P, Gormus J, Kaplan ME. Human monocyte-induced tumor cell cytotoxicity. Blood. 1978;52:211. [PubMed] [Google Scholar]
  • 30.Sone S, Tsubura E. Human alveolar macrophages: potentiation of their tumoricidal activity by liposome-encapsulated muramyl dipeptide. J Immunol. 1982;129:1313. [PubMed] [Google Scholar]
  • 31.Sone S, Moriguchi S, Shimizu E, Ogushi F, Tsubura E. In vitro generation of tumoricidal properties in human alveolar macrophages following interaction with endotoxin. Cancer Res. 1982;42:2227. [PubMed] [Google Scholar]
  • 32.Stewart CC, Beetham KC. Cytocidal activity and proliferative ability of macrophages infiltrating the EMT6 tumor. Int J Cancer. 1978;22:152. doi: 10.1002/ijc.2910220208. [DOI] [PubMed] [Google Scholar]
  • 33.Swinburne S, Cole PJ. Human peripheral blood monocyte and bronchoalveolar macrophage cytotoxicity for cultured human lung tumor cells. J Reticuloendothel Soc. 1982;32:371. [PubMed] [Google Scholar]
  • 34.Swinburne S, Moore M, Cole P. Human bronchoalveolar macrophage cytotoxicity for cultured human lung tumour cells. Br J Cancer. 1982;46:625. doi: 10.1038/bjc.1982.247. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 35.van Furth R, Blusse van oud Alblas A. The current view on the origin of pulmonary macrophages. Pathol Res Pract. 1982;175:38. doi: 10.1016/S0344-0338(82)80041-1. [DOI] [PubMed] [Google Scholar]

Articles from Cancer Immunology, Immunotherapy : CII are provided here courtesy of Springer

RESOURCES