Abstract
Previous studies by Prehn demonstrated a direct correlation between the dose of carcinogen used for tumor induction and the immunogenicity of the resulting tumors. The purpose of the present study was to determine the role of the host's immune response and the influence of the carcinogen on immune function in this relationship. For that reason, a comparison was made of the immunogenicities of tumors induced with two doses of carcinogen in immunologically normal mice and in mice immunodepressed by adult thymectomy and irradiation. If the direct relationship between dose and immunogenicity demonstrated in normal mice was due to the degree of immunosuppression produced by the carcinogen, this correlation should not be apparent in mice already immunosuppressed. Although there was some increase in the immunogenicity of tumors induced in the immunosuppressed mice, the same relationship between carcinogen dose and immunogenicity was observed in both groups of mice. These results indicate that the degree of immunogenicity of tumors induced with both high and low doses of carcinogen was influenced by immunoselection, but in addition another, non-immunologic factor was significant in the relationship between carcinogen dose and immunogenicity.
Keywords: Immune Response, Cancer Research, Immune Function, Direct Relationship, Normal Mouse
Footnotes
Abbreviations used: B6, C57BL/6J; CBy, BALB/cByJ; IP, intraperitoneal; IR, Immunogenicity Ratio; IV, intravenous; LP, latent period; MCA, 3-methylcholanthrene; SC, subcutaneous; TXB, adult thymectomized, lethally irradiated, and injected with 2×106 syngeneic bone marrow cells; TXR, thymectomized and sublethally irradiated; WBI, whole-body irradiation
References
- 1.Bartlett GL. Effect of host immunity on the antigenic strength of primary tumors. J Natl Cancer Inst. 1972;49:493. [PubMed] [Google Scholar]
- 2.Dixon FJ, Talmage DW, Maurer PH. Radiosensitive and radioresistant phases in the antibody response. J Immunol. 1952;68:693. [PubMed] [Google Scholar]
- 3.Embleton MJ, Heidelberger CL. Antigenicity of clones of mouse prostate cells transformed in vitro. Int J Cancer. 1972;9:8. doi: 10.1002/ijc.2910090103. [DOI] [PubMed] [Google Scholar]
- 4.Globerson A, Feldman M. Antigenic specificity of benzo [a]pyrene-induced sarcomas. J Natl Cancer Inst. 1964;32:1229. doi: 10.1093/jnci/32.6.1229. [DOI] [PubMed] [Google Scholar]
- 5.Johnson S. The effect of thymectomy and the dose of 3-methylcholanthrene on the induction and antigenic properties of sarcomas in C57BL mice. Br J Cancer. 1968;22:93. doi: 10.1038/bjc.1968.13. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 6.Lawler EM, Outzen HC, Prehn RT. Effect of different immunization and challenge procedures on in vivo tumor immunogenicity tests. Cancer Immunol Immunother. 1981;11:87. [Google Scholar]
- 7.Mondal S, Iype PT, Griesbach LM, Heidelberger C. Antigenicity of cells derived from mouse prostate cells after malignant transformation in vitro by carcinogenic hydrocarbons. Cancer Res. 1970;30:1593. [PubMed] [Google Scholar]
- 8.Murasko DM. Apparent lack of H-2 restriction of allograft rejection. J Immunol. 1978;121:958. [PubMed] [Google Scholar]
- 9.Old LJ, Boyse EA, Clarke AD, Carswell EA. Antigenic properties of chemically induced tumors. Ann NY Acad Sci. 1962;101:80. [Google Scholar]
- 10.Outzen HC, Ross SE, Prehn RT. Proceedings of the First International Workshop on Nude Mice. Stuttgart: Fischer; 1974. Cell-mediated immunologic responses of germ-free nude mice; p. 209. [Google Scholar]
- 11.Prehn RT. Function of depressed immunologic reactivity during carcinogenesis. J Natl Cancer. 1963;31:791. [PubMed] [Google Scholar]
- 12.Prehn RT. The relationship of immunology to carcinogenesis. Ann NY Acad Sci. 1969;164:449. doi: 10.1111/j.1749-6632.1969.tb14059.x. [DOI] [PubMed] [Google Scholar]
- 13.Prehn RT. In: Immune surveillance. Smith RT, Landy M, editors. New York: Academic Press; 1970. p. 451. [Google Scholar]
- 14.Prehn RT. Relationship of tumor immunogenicity to concentration of the oncogen. J Natl Cancer Inst. 1975;55:189. doi: 10.1093/jnci/55.1.189. [DOI] [PubMed] [Google Scholar]
- 15.Siegel S. Nonparametric statistics for the behavioral sciences. New York: McGraw-Hill; 1956. [Google Scholar]
- 16.Stjernsward J. Immunodepressive effect of 3-methylcholanthrene. Antibody formation at the cellular level and reaction against weak antigenic homografts. J Natl Cancer Inst. 1965;35:885. [PubMed] [Google Scholar]
- 17.Stjernsward J. Further immunological studies of chemical carcinogenesis. J Natl Cancer Inst. 1967;38:515. [PubMed] [Google Scholar]
- 18.Stutman O. Immunodepression and malignancy. Adv Cancer Res. 1975;22:261. doi: 10.1016/s0065-230x(08)60179-7. [DOI] [PubMed] [Google Scholar]