Skip to main content
NCBI home page
Cancer Immunology, Immunotherapy : CII logoLink to Cancer Immunology, Immunotherapy : CII
. 1984 Jun;17(1):28–32. doi: 10.1007/BF00205493

Cell-mediated immune reaction against BK virus-transformed cells

Rauno Mäntyjärvi 1,✉, Henry Karjalainen 1, Mariitta Laaksonen 1
PMCID: PMC11039232  PMID: 6203636

Abstract

Syngeneic or allogeneic cells transformed by BK virus (BKV) were used to immunize C57BL/6J mice. After in vitro stimulation, lymphocytes prepared from the spleens of immunized mice were used in in vitro cytotoxicity tests. The results of these tests revealed the presence of a cell surface antigen, presumably corresponding to the viral transplantation antigen, common to all tested BKV- and SV40-transformed cell lines of C57BL/6J origin. An allogeneic cell line transformed by BKV also contained the same antigen. Immunization, i.e., in vivo priming, did not require syngeneic transformed cells, whereas cytolysis was only observed when the virus-specific antigen on target cells was associated with the same H2 haplotype as was expressed by effector cells. An additional unidentified antigen was shared by some of the BKV-transformed cell lines and cell lines transformed by simian adenovirus SA7.

Keywords: Immune Reaction, Cell Surface, Target Cell, Effector Cell, Surface Antigen

References

  • 1.Anderson JL, Martin RG, Chang C, Mora PT, Livingston DM. Nuclear preparation of SV40-transformed cells contain tumor-specific transplantation antigen activity. Virology. 1977;76:420. doi: 10.1016/0042-6822(77)90314-2. [DOI] [PubMed] [Google Scholar]
  • 2.Barra Y, Ito Y, Depetris D, Meyer G. Characterization of tumor-specific transplantation antigen induced in cells infected or transformed by polyomaviruses. J Natl Cancer Inst. 1983;70:283. [PubMed] [Google Scholar]
  • 3.Beth E, Giraldo G, Schmidt-Ullrich R, Pater MM, Pater A, diMayorca G. BK virus-transformed inbred hamster brain cells. I. Status of the viral DNA and the association of BK virus early antigens with purified plasma membranes. J Virol. 1981;40:276. doi: 10.1128/jvi.40.1.276-284.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 4.Burton RC, Chism SE, Warner NL. In vitro induction of tumor-specific immunity. VII. Does autosensitization occur with in vitro culture of T lymphocytes? J Immunol. 1977;119:1329. [PubMed] [Google Scholar]
  • 5.Campbell AE, Foley FL, Tevethia SS. Demonstration of multiple antigenic sites of the SV40 transplantation rejection antigen by using cytotoxic T lymphocyte clones. J Immunol. 1983;130:490. [PubMed] [Google Scholar]
  • 6.Chang C, Chang R, Mora PT, Hu C-P. Generation of cytotoxic lymphocytes by SV40-induced antigens. J Immunol. 1982;128:2160. [PubMed] [Google Scholar]
  • 7.Deppert W, Pates R. Cell surface location of simian virus 40-specific proteins on Hela cells infected with adenovirus type 2-simian virus 40 hybrid viruses Ad2+ ND1 and Ad2+ND2. J Virol. 1979;31:522. doi: 10.1128/jvi.31.2.522-536.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 8.Dorfman N, Winkler D, Burton RC, Kossayda N, Sabia P, Wunderlich J. Broadly reactive murine cytotoxic cells induced in vitro under syngeneic conditions. J Immunol. 1982;129:1762. [PubMed] [Google Scholar]
  • 9.Dougherty RM. A comparison of human papovavirus T antigens. J Gen Virol. 1976;33:61. doi: 10.1099/0022-1317-33-1-61. [DOI] [PubMed] [Google Scholar]
  • 10.Forni G, Green I. Heterologous sera: A target for in vitro cell-mediated cytotoxicity. J Immunol. 1976;116:1561. [PubMed] [Google Scholar]
  • 11.Gooding LR. Specificities of killing by cytotoxic lymphocytes generated in vivo and in vitro to syngeneic SV40-transformed cells. J Immunol. 1977;118:920. [PubMed] [Google Scholar]
  • 12.Gooding LR, Edwards CB. H-2 antigen requirements in the in vitro induction of SV40-specific cytotoxic T lymphocytes. J Immunol. 1980;124:1258. [PubMed] [Google Scholar]
  • 13.Grossi MP, Caputo A, Meneguzzi G, Corallini A, Carra L, Portolani M, Porgatti M, Milanesi G, Barbanti-Brodano G. Transformation of human embryonic fibroblasts by BK virus, BK virus DNA and a subgenomic BK virus DNA fragment. J Gen Virol. 1982;63:393. doi: 10.1099/0022-1317-63-2-393. [DOI] [PubMed] [Google Scholar]
  • 14.Habel K. Resistance of polyoma virus-immune animals to transplanted polyoma tumors (26453) Proc Soc Exp Biol Med. 1961;106:722. doi: 10.3181/00379727-106-26453. [DOI] [PubMed] [Google Scholar]
  • 15.Haran-Ghera N, Peled A. Induction of leukemia in mice by irradiation and radiation leukemia virus variants. Adv Cancer Res. 1979;30:45. doi: 10.1016/s0065-230x(08)60894-5. [DOI] [PubMed] [Google Scholar]
  • 16.Howell SB, Dean JH, Law LW. Defects in cell-mediated immunity during growth of a syngeneic simian virus-induced tumor. Int J Cancer. 1975;15:152. doi: 10.1002/ijc.2910150118. [DOI] [PubMed] [Google Scholar]
  • 17.Imamura M, Martin WJ. Variation in histocompatibility antigen expression on murine tumors: Differences in expression and immunogenicity of H-2K region-coded unique antigens. J Immunol. 1982;129:877. [PubMed] [Google Scholar]
  • 18.Ismail A, Baumann EA, Hand R. Cell surface T antigen in cells infected with simian virus 40 or an adenovirus-simian virus 40 hybrid, Ad2+ D2. J Virol. 1981;40:615. doi: 10.1128/jvi.40.2.615-619.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 19.Jay G, Jay FT, Chang C, Friedman RM, Levine AS. Tumor-specific transplantation antigen: Use of the Ad2+ND1 hybrid virus to identify the protein responsible for simian virus 40 tumor rejection and its genetic origin. Proc Natl Acad Sci USA. 1978;75:3055. doi: 10.1073/pnas.75.7.3055. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 20.Karjalainen HE, Laaksonen AM, Mäntyjärvi RA. Tumour-specific transplantation antigen in hamster tumour cells induced with BK virus. J Gen Virol. 1978;41:171. doi: 10.1099/0022-1317-41-1-171. [DOI] [PubMed] [Google Scholar]
  • 21.Karjalainen HE, Laaksonen AM, Mäntyjärvi RA. Virus-specific antigens in BK virus-transformed cells detected by cell-mediated immune reactions. Med Biol. 1983;61:73. [PubMed] [Google Scholar]
  • 22.Kato K, Iwamura Y, Kurimura T. Induction of SV40-related transplantation immunity in mice by BK virus-transformed cells. Jpn J Med Sci Biol. 1979;32:311. doi: 10.7883/yoken1952.32.311. [DOI] [PubMed] [Google Scholar]
  • 23.Khera KS, Ashkenazi A, Rapp F, Melnick JL. Immunity in hamsters to cells transformed in vitro and in vivo by SV40. Tests for antigenic relationship among the papovaviruses. J Immunol. 1963;91:604. [PubMed] [Google Scholar]
  • 24.Knowles BB, Koncar M, Pfizenmaier K, Solter D, Aden DP, Trinchieri G. Genetic control of the cytotoxic T cell response to SV40 tumor-associated specific antigen. J Immunol. 1979;122:1798. [PubMed] [Google Scholar]
  • 25.Law LW, Takemoto KK, Rogers MJ, Henriksen O, Ting RC. Differences in the capacity of simian virus 40 (SV40) tumor antigens on cells, membranes and in soluble form to induce transplantation immunity in hamsters and mice. Int J Cancer. 1978;22:315. doi: 10.1002/ijc.2910220315. [DOI] [PubMed] [Google Scholar]
  • 26.Lewis AM, Rowe WP. Studies of nondefective adenovirus 2-simian virus 40 hybrid viruses. VIII. Association of simian virus 40 transplantation antigen with a specific region of the early viral genome. J Virol. 1973;12:836. doi: 10.1128/jvi.12.4.836-840.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 27.Padgett BL, Hunt JM, Walker DL. Specificity of the tumor-specific transplantation antigen induced by JC virus, a human polyomavirus. Intervirology. 1977;8:182. doi: 10.1159/000148893. [DOI] [PubMed] [Google Scholar]
  • 28.Pater MM, Pater A, Blashka K, diMayorca G (1982) BK virus and its variants: Association with human tumors. In: Proceedings of the 13th International Cancer Congress, Seattle, Washington USA, p 432
  • 29.Pross HF, Luk SS, Baines MG. Spontaneous human lymphocyte-mediated cytotoxicity against tumor target cells. V. The role of serum-derived heterologous membrane antigens. Int J Cancer. 1978;21:291. doi: 10.1002/ijc.2910210307. [DOI] [PubMed] [Google Scholar]
  • 30.Purchio AF, Fareed GC. Transformation of human embryonic kidney cells by human papovavirus BK. J Virol. 1979;29:763. doi: 10.1128/jvi.29.2.763-769.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 31.Rassart E, Sankar-Mistry P, Lemay G, DesGroseillers L, Jolicoeur P. New class of leukemogenic ecotropic recombinant murine leukemia virus isolated from radiation-induced thymomas of C57BL/6 mice. J Virol. 1983;45:565. doi: 10.1128/jvi.45.2.565-575.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 32.Rogers MJ, Law LW, Appella E. Subcellular distribution of the tumor-specific transplantation antigen of simian virus 40-transformed cells. J Natl Cancer Inst. 1977;59:1291. doi: 10.1093/jnci/59.4.1291. [DOI] [PubMed] [Google Scholar]
  • 33.Russell WC, Newman C, Williamson DH. A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses. Nature. 1975;253:461. doi: 10.1038/253461a0. [DOI] [PubMed] [Google Scholar]
  • 34.Seehafer J, Downer DN, Gibney DJ, Colter JS. Evidence for the expression of TSTA in BKV-transformed cells: Cross-reaction with SV40 TSTA. Virology. 1979;95:241. doi: 10.1016/0042-6822(79)90423-9. [DOI] [PubMed] [Google Scholar]
  • 35.Shah KV, Daniel RW, Strandberg JD. Sarcoma in a hamster inoculated with BK virus, a human papovavirus. J Natl Cancer Inst. 1975;54:945. [PubMed] [Google Scholar]
  • 36.Simmons DT, Martin MA. Common methionine-tryptic peptides near the amino-terminal end of primate papovavirus tumor antigens. Proc Natl Acad Sci USA. 1978;75:1131. doi: 10.1073/pnas.75.3.1131. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 37.Soule HR, Lanford RE, Butel JS. Antigenic and immunogenic characteristics of nuclear and membrane-associated simian virus 40 tumor antigen. J Virol. 1980;33:887. doi: 10.1128/jvi.33.2.887-901.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 38.Stephenson JR, Greenberger JS, Aaronson SA. Oncogenicity of an endogenous C-type virus chemically activated from mouse cells in culture. J Virol. 1974;13:237. doi: 10.1128/jvi.13.1.237-240.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 39.Takemoto KK, Mullarkey MF. Human papovavirus, BK strain: Biological studies including antigenic relationship to simian virus 40. J Virol. 1973;12:625. doi: 10.1128/jvi.12.3.625-631.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 40.Takemoto KK, Linke H, Miyamura T, Fareed GC. Persistent BK papovavirus infection of transformed human fetal brain cells. I. Episomal viral DNA in cloned lines deficient in T-antigen expression. J Virol. 1979;29:1177. doi: 10.1128/jvi.29.3.1177-1185.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 41.Tevethia SS. Immunology of simian virus 40. In: Klein G, editor. Viral oncology. New York: Raven Press; 1980. p. 581. [Google Scholar]
  • 42.Tevethia SS, Blasecki JW, Waneck G, Goldstein AL. Requirement of thymus-derived ϑ-positive lymphocytes for rejection of DNA virus (SV40) tumors in mice. J Immunol. 1974;113:1417. [PubMed] [Google Scholar]
  • 43.Tevethia SS, Flyer DC, Tijian R. Biology of simian virus (SV40) transplantation antigen (TrAg). VI. Mechanism of induction of SV40 transplantation immunity in mice by purified SV40 T antigen (D2 protein) Virology. 1980;107:13. doi: 10.1016/0042-6822(80)90268-8. [DOI] [PubMed] [Google Scholar]
  • 44.Trincieri G, Aden DP, Knowles BB. Cell-mediated cytotoxicity to SV40-specific tumour-associated antigens. Nature. 1976;261:312. doi: 10.1038/261312a0. [DOI] [PubMed] [Google Scholar]

Articles from Cancer Immunology, Immunotherapy : CII are provided here courtesy of Springer

RESOURCES