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. 2024 Mar 28;14(4):191. doi: 10.3390/metabo14040191

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Identification of differentially abundant lipids in the diet treatment group (n = 20) at baseline compared to Week 8 (post-intervention). (A) Boxplots illustrating all matched paired measurements (baseline and Week 8) for each lipid class, including the median and interquartiles. Paired sample t-tests were used to identify significant differences (p < 0.05) between the means of the matched pairs. Statistically significant p-values are shown in the figure. (B) Supervised PLS-DA analysis of lipidomic data. The first two latent components (Comp 1 and Comp 2) are presented with 95% confidence ellipses surrounding the cluster core. (C) VIP plot illustrating the average normalized values of the top 20 VIP scores from the PLS-DA analysis. (D) Volcano plot showing the log₂ fold change versus the −log₁₀(p-value) for pairwise comparisons. Lipid species that significantly increased in abundance are shown in red, while those that significantly decreased are indicated in blue.

Identification of differentially abundant lipids in the diet treatment group (n = 20) at baseline compared to Week 8 (post-intervention). (A) Boxplots illustrating all matched paired measurements (baseline and Week 8) for each lipid class, including the median and interquartiles. Paired sample t-tests were used to identify significant differences (p < 0.05) between the means of the matched pairs. Statistically significant p-values are shown in the figure. (B) Supervised PLS-DA analysis of lipidomic data. The first two latent components (Comp 1 and Comp 2) are presented with 95% confidence ellipses surrounding the cluster core. (C) VIP plot illustrating the average normalized values of the top 20 VIP scores from the PLS-DA analysis. (D) Volcano plot showing the log₂ fold change versus the −log₁₀(p-value) for pairwise comparisons. Lipid species that significantly increased in abundance are shown in red, while those that significantly decreased are indicated in blue.