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. 2024 Apr 11;13(4):310. doi: 10.3390/pathogens13040310

Table 2.

RT-qPCR primers for immune-related genes.

Genes Accession NO. Primers References
18S ribosomal RNA AF021880 F-GAGAAACGGCTACCACATCC
R-GATACGCTCATTCCGATTACAG
[26]
CD4-1 DQ435305 F-GATGTCATCATTGTAGATCTCG
R-GAGGTAGCTGGCATTTCACTCC
[27]
CD4-2 DQ435304 F-CTGTATGTTGTATCAGCCTCTG
R-CAGTCACCTCCTTACTTTGGCTA
[27]
CD8-α HQ446239 F-CTACGCGGAGAGACAGTCCCAA
R-CTCACAACCCAAAAGCACATC
[27]
CD8-β HQ446240 F-CCATCAGGCCTGGAGAAAGCA
R-TCACCACCAGGAGTAGGACA
[27]
IL-1β DQ157743 F-TGATCCTTTGGCCATGAGCGGC
R-AGACATTGAAAAGCTCCTGGTC
[27]
TLR-4 x79482 F-ACCTGACTACCACACCCATA
R-TCCTAGACGAGTGGAGGTTATT
This study
TLR-5 x79482 F-GGAAGCGCTACAAATCCTACT
R-GTATGCCAGATCAAGTCGTATCA
This study
INFγ NC_030434 F-TTGGGCAAAGTAGAGGACACC
R-TGTTTCCACACTGCCTGTTCG
[27]
MHC class II AF103002 F-GACACCAGGACATGGGAGGTG
R-CGAGGAAGAAAGTTCCGGTAG
[27]
MHC class I I AF103 F-GACCGAGAACTCGACTACTACA
R-GAGTGCCTTTCTCCCAGTAATC
This study
TNFα AJ417565 F-GCACAACAAACCAGACGAGA
R-TCGTTGTCCTCCAGTTTCAA
[27]
IgM x79482 F-AAGAAGCGAGTTATGCACCAG
R-ATGCTTCATGTTCCACCTCAC
This study