Figure 2.

ITIH3 lowers liver triglyceride accumulation both in vivo and in vitro

Comparisons of body weight measurements and hepatic lipid levels from (A and B) 15% fructose in drinking water or (C and D) HF/HS fed ITIH3 silencing (KD) mice or (E and F) HF/HS fed ITIH3 overexpressing (OEx) mice, respectively. Oil Red O staining of AML12 cells overexpressing GFP or ITIH3 with (G) representative images and (H) quantification. Data are presented as mean ± SEM (n = 6–8 mice per group; n = 2 independent experiments for AML12 cells). p values were calculated by (A, C, and E) repeated measures two-factor ANOVA; (B, D, and F) two-factor ANOVA corrected by post-hoc “Holm-Sidak’s” multiple comparisons test; (H) t test. ∗p < 0.05; ∗∗∗p < 0.001. TG, triglyceride; TC, total cholesterol; UC, unesterified cholesterol; PL, phospholipids.