Fig. 1.

Adipocyte hypertrophy and hyperplasia in patients with obesity. (a) Average adipocyte area quantification in the WAT of lean patients (N = 9), patients with obesity (N = 61) and patients with obesity and T2DM (N = 27). (b) Representative H&E staining of omental WAT with presence of small adipocytes and large adipocytes in patients with obesity with and without T2DM. (c) Western Blot of WAT PPARγ and CEBP⍺ in patients with obesity with and without T2DM stratified according to the adipocyte size (N = 3 per group). (d) Correlation between adipocyte area (μm²) and BMI (kg/m²; left panel) and adipocyte area (μm²) and plasma glycated haemoglobin [HbA1c (mmol/mol); right panel]. Statistical significance was calculated using ANOVA with Tukey's multiple comparison test for (a) and (c) and using Pearson correlation coefficient for (d). Error bars represent SEM. Scale bars; 250 microns.