Skip to main content
. 2024 Apr 23;13(9):734. doi: 10.3390/cells13090734

Figure 8.

Figure 8

Metabolomic analysis of polar metabolites in plasma samples 7 days after controlled cortical impact (CCI) or sham surgery in animals treated with vehicle or VPA (3 dpi 400 mg/kg VPA i.p.). (A) Volcano plot of CCI-Vehicle versus CCI-VPA. Metabolites that were increased in the VPA group are on the right side of the x-axis (sham groups in Supplementary Figure S4B). (B) Partial least square discriminant analysis (PLS-DA) component 1 versus component 2 scatter plot and 85% confidence ellipses. One VPA animal each in the CCI and sham group were excluded, which were identified as outliers by random forest analysis. (C) Variable importance plot (VIP) of PLS-DA component-1. (D) XY scatter plots showing the association of key regulated metabolites versus Il1b. Associations with Tnfa are shown in Supplementary Figure S6B. Sample sizes: controlled cortical impact (CCI) vehicle n = 12, CCI-VPA n = 12, sham vehicle n = 8, sham-VPA n = 8.