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Primary cultures can be derived from various tissues including skin, blood, neurons, and urine. Cellular reprogramming for iPSC generation can be conducted using different combinations of transcription factors, with the Yamanaka factors (OSKM) remaining the most commonly employed. Similarly, there are diverse tools available to facilitate cell transfection for reprogramming, such as MMLV, SENDAI virus, liposome, and transposons.
