Figure 2. Sex and total adolescent alcohol consumption only modestly increase total alcohol consumption during adulthood DID in mice.

(A) Experimental timeline. (B) Neither Sex nor adolescent DID exposure affect EtOH consumption on first adulthood 2-hour exposure at PND 84. (C) Female mice drank more than male mice during the first 4-hour binge session at PND 87, but this effect was not modulated by adolescent DID. (D) There were main effects of sex and adolescent DID on total EtOH consumption across all 16 days of adulthood DID. (E) Adolescent DID experience did not affect BECs achieved during the final adulthood binge session (n = 4 Adol H2O mice, n = 3 Adol DID mice, sexes combined). (F-H) Relationships between total adolescent EtOH consumption and each of the 3 adult binge drinking parameters – EtOH consumed in first 2-hour exposure, first 4-hour binge, and across all 16 sessions of DID. Significant predictors identified by GLME are indicated where appropriate. (I) Daily EtOH consumption during adulthood DID in female (top) and male (bottom) mice. (J) Adolescent DID did not affect body weight in female (top) or male (bottom) mice. (K) Female and male Adolescent DID mice do not change their drinking during the first 2-hour exposure in adolescence (PND 28) compared to in adulthood (PND 84). (L) There was no difference in EtOH consumption during first binge day in adolescence (PND 31) compared to adulthood (PND 87). (M) While female mice do not show changes in total EtOH consumption in adolescence and adulthood DID, male mice show reduced total EtOH intake during adulthood DID. (N) Daily EtOH consumption in adolescent and adulthood DID in double drinking mice. Trendlines in F-H represent the line of best fit within each sex. Significant main effects are indicated as * = p < .05, **** = p < .0001