Table 1:
gRNAs | |
RB1 crRNA | 5’- AGAGAGAGCUUGGUUAACUU -3’ |
Primers for amplification | |
PGBD5 forward | 5’- CTCTGGGTCAGACAATGTTCTTC -3’ |
PGBD5 reverse | 5’- GCTTATTCTTCAGCGCATCCA -3’ |
GAPDH forward | 5’- GATCATCAGCAATGCCTCCT −3’ |
GAPDH reverse | 5’- GTCATGAGTCCTTCCACGATAC −3’ |