Display of proteins on Bacillus subtilis endospores

Abstract

The targeting and anchoring of heterologous proteins and peptides to the outer surface of bacteriophages and cells is becoming increasingly important, and has been employed as a tool for fundamental and applied research in microbiology, molecular biology, vaccinology, and biotechnology. Less known are endospores or spores produced by some Gram-positive species. Spores of Bacillus subtilis are surrounded by a spore coat on their outside, and a few proteins have been identified being located on the outside layer and have been successfully used to immobilize antigens and some other proteins and enzymes. The major advantage of spores over the other published systems is their synthesis within the cytoplasm of the bacterial cell. Therefore, any heterologous protein to be anchored on the outside does not have to cross any membrane. Furthermore, spores are extremely resistant against high temperature, irradiation and many chemicals, and can be stored for many years at room temperature.

Introduction

Surface display is a powerful technique that uses natural microbial functional components to express heterologous peptides and proteins on the exterior of phages or cells. The first surface expression system was developed by George P. Smith who used the gene III of the filamentous phage M13 [1] for display. This gene codes for the protein pIII which is present in 5–8 copies near one end of the phage particle [2, 3]. The pIII protein consists of two domains separated by a linker region. Smith published the pioneering idea to insert parts of the coding region of an endonuclease gene in-frame in the linker region followed by the identification of the recombinant phages using appropriate antibodies in a technique termed biopanning [1]. This technique is now well known as surface display of peptides and proteins on the surface not only of phages but also on whole cells of bacteria and yeast. Besides the filamentous phages, display systems have also been developed for bacteriophages, λ, e.g., [4–6], T4, e.g., [6–8], and T7 [9]. The concept of using naturally occurring surface proteins as an anchor for targeting proteins of interest (often called passenger proteins), with a distinct function at the surface of phages and cells summarized as bioparticles, offers a broad range of applications in many different areas of bioscience, including the screening of novel binding partners, delivery of vaccines and drugs, production of active enzymes and antibodies for cleanup of industrial and environmental pollution, and their use as biosensors, biocatalysts for bioconversion, and screening of peptides libraries [6–8]. All these systems share a common theme, targeting recombinant proteins to the phage or cell surface by constructing gene fusions using sequences from membrane-anchored domains of surface or phage coat proteins.

The polypeptides displayed at the particle surface are freely accessible to the substrate or binding partner in activity and binding studies. Furthermore, proteins have been proven to be more stable when connected to a bioparticle rather than as free, diffusible molecules, and this makes it unnecessary to purify these proteins. There are several excellent review articles dealing with the application of surface display using bacterial cells and bacteriophages [10–20].

When filamentous phages or bacterial cells are used for surface display, the chimeric proteins have to cross at least one membrane, the cytoplasmic membrane. For obvious reasons, not every chimeric protein is able to cross this membrane. Those proteins which either fold fast in the cytoplasm or which contain hydrophobic patches provoking entrapment in the membrane are refractory to translocation through the cytoplasmic membrane. All these problems can be prevented by using endospores formed by Bacillus and related aerobic endospore-forming bacteria (a group of some 200 species, distributed over 25 genera) as well as by Clostridium as a strategy to survive during unfavorable conditions. Since these spores are formed within the sporulating cells, the mother cells, Bacillus offers four advantages. First, the chimeric proteins do not have to cross the cytoplasmic membrane and also fast folding passenger proteins should be anchored on the spore surface. Furthermore, the full set of ATP-dependent molecular chaperones is present to assist the correct folding of the passenger protein if necessary. Third, proteins being multimers to become active or proteins that need an intracellular co-factor (for example, transaminase with pyrodoxal phosphate as a co-factor) are other challenging targets for spore surface display. And fourth, it should even be possible to obtain formation of disulfide bonds by providing the appropriate conditions within the cytoplasm as has been published for E. coli [21, 22]. In the following chapters, we will first review the most relevant data leading to spore formation in B. subtilis, the spore composition and the germination process. Then, the current state of spore surface display will be reviewed.

Formation of the B. subtilis endospore

When confronted by nutrient depletion, the normally rod-shaped B. subtilis cells produce an oval dormant cell, the spore. The molecular and biochemical reactions underlying the genetic program resulting in spore formation have been studied most extensively in B. subtilis, but all members of the two groups Bacillus and Clostridium produce endospores according to a program which is similar to that of B. subtilis [23–25]. Bacterial endospores are commonly found in any soil and water environment, but they can colonize virtually any habitat. They are entirely distinct from the vegetative cell, possessing several molecular and cellular structures seen nowhere else in nature. These unique components contribute to the spore’s most striking characteristics. It is metabolically inactive, highly resilient to environmental assault, and stable for extreme periods of time. Spores are the most resistant form of life on earth, and they can withstand extremes of heat, radiation, chemical assault, and time [26, 27]. Viable spores were recovered from the gut of a bee fossilized in Dominican amber for an estimated 25–40 million years [28]. But the spore can sense the reappearance of even minute amounts of nutrients in the environment, and respond by converting back to a vegetative growing cell [29]. Very recently, it has been published that muropeptides also act as potent germinants [30]. This process leading to the conversion of spores to vegetative cells is called germination (see below).

Five different kinases, KinA through KinE [31], sense so far unknown starvation factors, autophosphorylate, and transfer the phosphoryl group through two proteins, Spo0F and Spo0B, to the master regulator Spo0A [32]. This process is called phosphorelay, and Spo0A~P acts both as a transcriptional repressor and activator to regulate transcription of a total of 121 genes [33]. A major function of Spo0A~P is to activate expression of genes encoding two sporulation-specific sigma factors. As sporulation proceeds, there is an unequal cell division that generates a large mother cell and a smaller forespore (Fig. 1). The plasma membrane of the mother cell then grows around the forespore, generating an engulfed forespore surrounded by two membranes. Activation and/or synthesis of additional sigma factors in a compartment-specific manner then drive different patterns of gene expression in the mother cell and in the forespore compartment. The subsequent synthesis of a thick peptidoglycan cortex between the outer and inner forespore membrane is accompanied by a large decrease in the volume and water content of the forespore protoplast, and there is also a decrease in the forespore pH [34]. The forespore later takes up tremendous amounts of pyridine-2,6-dicarboxylic acid (dipicolinic acid, DPA) that has been synthesized in the mother cell, which decreases the forespore water content even further (see [35–37] for recent reviews).

Fig. 1.

Stages of sporulation. a Vegetative cell in the phase 0; b cell at stage II where the asymmetric septum has been formed; c cell at stage III where the cytoplasmic membrane of the mother cell has engulfed the forespore; d cell at stage IV where formation of the coat has already started; the spore is about to be released from the lysed mother cell. Figure adapted from [104]

Composition of the B. subtilis endospore

The Bacillus spore consists of three concentric compartments separated by two membranes (Fig. 2). The central part has been designated the spore core. It contains one copy of the bacterial chromosome and plasmid copies if present in the vegetative cell. The DNA molecules are complexed with small acid-soluble proteins (SASPs), the predominant proteins of the core making up as much as 20% of the total spore protein [38]. The most abundant SASPs fall into one of three classes, based on sequence comparisons and biochemical properties: α, β, and γ [39]. The SASPs protect spores from a wide range of damaging agents, including heat, UV radiation, nucleases, hydroxyl radicals, formaldehyde, nitrous acid nucleases and desiccation [40]. The α/β-type SASPs protect the spore by binding to and saturating its DNA [41]. These proteins do not possess any known DNA-binding motifs, and their interaction with DNA is relatively non-specific. Upon binding to DNA, the α/β-type SASPs alter DNA conformation locally, stiffening the DNA and changing it from a B to an A conformation [42]. Besides the SASPs, the dehydrated state of the core and ions such as DPA, mainly as calcium chelate [43], also play critical roles. A second function of the SASPs is to serve as a source of amino acids after proteolysis, toward the end of germination (see below). Furthermore, the normal cellular cytoplasmic components, from RNA, enzymes to ribosomes, are present, but all in an inactive state.

Fig. 2.

Structure of the spore showing the different compartments. The innermost compartment, the core, contains the DNA and is filled with small acid-soluble proteins that saturate the DNA and protect it and all the other components present in the cytoplasm in a largely dehydrated state. The spore core is surrounded by two membranes sandwiching a specialized peptidoglycan called cortex keeping the water activity relatively low. The spore coat consists of two layers and is composed of about 70 different proteins. Pathogenic and some saprophytic Bacilli possess an additional layer called exosporium consisting of a paracrystalline basal layer and a hair-like nap. A thin section electron micrograph showing a spore with its exosporium has been presented [108]

The inner membrane of the dormant spore is in an unusual environment and has been described as ‘semi-crystalline’, only regaining the fluidity characteristics of a normal cell membrane when the spore germinates [44]. It has a low permeability to small molecules, perhaps even water [45]. The low permeability of this membrane is one factor in the resistance of spores to some chemicals, in particular DNA-damaging chemicals [40].

The surface of the inner spore membrane is the site of assembly of a thin layer of peptidoglycan called the primordial germ cell wall, similar in composition to the vegetative cell wall that serves as the primordial wall of the newly formed vegetative cell following spore germination (Fig. 2). The outer prespore membrane is the site of assembly of a second, thicker, and chemically distinct layer of peptidoglycan called the spore cortex, which is essential for the attainment and maintenance of the dehydrated state of the spore core, for spore mineralization and for dormancy [27, 46, 47]. The cortex beneath the outer spore membrane is composed predominantly of peptidoglycan with a structure similar to that of peptidoglycan in growing cells. However, it contains two modifications: muramic acid-δ-lactam and muramic acid linked only to alanine.

The third compartment, the spore coat, is a complex, multilayered assemblage of more than 50 proteins that encases the spore and helps to protect it from noxious environmental agents [46]. It is composed out of two major layers [26, 27]: a lightly staining, finally striated inner coat composed of several fine lamellae and a more darkly staining coarsely layered outer coat, made up of several thicker sublayers. The more than 50 different coat proteins range in size from about 6 to about 70 kDa, some of which have been subject to detailed analysis [46, 48, 49]. The assembling coat is tethered to this membrane by the 26-amino-acid SpoVM peptide, which is believed to form an amphipathic helix. It has been shown that proper localization of SpoVM is dependent on SpoIVA, a morphogenetic protein that forms the basement layer of the spore coat [50]. Conversely, proper localization of SpoIVA is dependent on SpoVM, and this mutual dependence is mediated in part by an amino acid side-chain located near the extreme C-terminus of SpoIVA and an amino acid side-chain on the hydrophilic face of the SpoVM helix. SpoIVA both binds and hydrolyses ATP and self-assembles into cable-like structures depending on ATP hydrolysis [51]. The coat apparently has a variety of functions. It has long been known to serve as a barrier against entry of large toxic molecules, such as a lysozyme, and to have a role in germination [26]. The most recent identification of a germination protein [52] in the coat should help to clarify its role in germination, and the discovery of coat protein oxidases [53–55] points to additional active roles that have yet to be elucidated.

Most coat proteins appear to be dispensable for the coat’s barrier functions, because the deletion of any one of the corresponding genes has no detectable consequence. Two important exceptions are SpoIVA and CotE. SpoIVA resides between the coat and the cortex [56, 57]. As this location suggests, SpoIVA connects the coat to the spore surface; without it, the coat is built but not attached to the spore [47, 58, 59]. CotE is positioned at the inner coat/outer coat interface [56]. It is responsible for the assembly of the entire outer coat as well as several inner coat proteins [60–63].

Germination of the B. subtilis endospore

Spores germinate in response to an environmental signal, and the most common germinants are amino acids, sugars or ribosides. This germination process uses components already established in the spore during its formation, and is essentially a biophysical and degradative one [64]. The germinant has to first penetrate the coat and cortex layers of the spore before coming in contact with the germinant receptors [65] located in the inner spore membrane [66]. In B. subtilis, these receptors are encoded by three homologous tricistronic operons termed gerA, gerB and gerK which are expressed in the forespore late in sporulation [64, 67]. While the GerA receptor recognizes l-alanine, the GerB and GerK receptors are involved for germination in the presence of a mixture of asparagine, glucose, fructose and K⁺ (AGFK). When spores become committed to germination, the following reactions will take place [64, 67].

First, the downstream consequences of germination-receptor interaction include a rapid efflux of monovalent cations (H⁺, Na⁺ and K⁺) and Zn²⁺, where the release of H⁺ elevates the core pH from ~6.5 to 7.7. Second, the large depot of DPA (about 10% of the spore dry weight) and its associated divalent cations (mainly Ca²⁺) will be released and replaced by water. Third, lytic enzymes that hydrolyze the specialized peptidoglycan of the cortex are activated, leading to full rehydration [52, 68–70].

In B. subtilis, one of the two germination-specific lytic enzymes (CwlJ) is localized at the coat–cortex interface, and can be activated artificially by high concentrations of external Ca²⁺-DPA, which might also be the natural activating signal for this enzyme [71]. The other major lytic enzyme, SleB, is present in the spore inner membrane and in the outer layers [68]. Both enzymes, CwlJ and SleB, play redundant roles in the degradation of the spore’s peptidoglycan cortex during germination. Therefore, the germination frequency in a double knockout is reduced by five orders of magnitude [72]. A similar reduction can be obtained by using the gerD-cwlB double knockout mutant. The SASPs are degraded by a specific protease, releasing DNA from its complexed state [67]. The metabolizing and energized germinated spore can then resume RNA, protein, and DNA synthesis in the outgrowth phase [73]. This further increase in core hydration will result in protein mobility and activation of enzyme activities. All these events take place without detectable energy metabolism. The return of enzymatic activities in the spore core allows initiation of spore metabolism, followed by macromolecular synthesis finally converting the germinated spore into a growing cell, and this period has been designated spore outgrowth.

Anchoring of passenger proteins on the endospore surface of B. subtilis

The spore-display system is based on the construction of gene fusions between heterologous DNA and a B. subtilis gene coding for a component of the spore coat, where the spore coat protein has been shown to be located on its outside. Three different spore coat proteins have been used to far, CotB, CotC, and CotG. All three proteins are tyrosine-rich coat components present as multimers within the spore coat [74, 75], and they are mainly if not exclusively associated with the outer coat [75–78]. The cotB gene encodes a 46-kDa polypeptide (CotB-46) which is posttranslationally converted into a form of about 66 kDa (CotB-66) [76]. CotC forms multimeric species with itself and the nearly identical CotU protein at the surface [75, 79]. CotG has a central region formed by nine tandem repeats of a lysine-, serine-, and arginine-rich motif [78]. While in the case of CotC and CotG the full-length versions are used as an anchoring motif, with CotB a shorter version is used [77].

Until now, major applications using B. subtilis spores are focused on delivery of surface-expressed antigens using CotB and CotC as anchoring motifs. Initial developments in this field have been summarized in three reviews [80–82]. Since then, additional antigens, detailed results about vaccination, immunogenicity, and the fate of spores within the animals have been published. The CotG protein has been used to anchor different enzymes on the spore surface. In the following sections, we will focus on various spore-based display systems and their applications as well as the initial developments in this field.

The first report of spore surface display was done with CotB as an anchoring motif and the coding region of TTFC (C-terminal fragment of the tetanus toxin), a well-characterized and highly immunogenic model antigen [83]. The 459-amino-acid TTFC was fused to the N-terminus, the truncated C-terminus of CotB, and was inserted into the middle of CotB. All three fusion proteins exhibited a similar surface location as confirmed by flow cytometry [84]. Next, spores displaying the C-terminal CotB-TTFC fusion were used for oral and intranasal immunization of mice. It turned out that these recombinant spores generated both a mucosal (IgA) and a systemic (IgG) response in the murine model [85]. In a further report, mice were inoculated intragastrically with spores displaying the CotB-TTFC, and a significant high serum TTFC-specific IgG response was stimulated [86]. The responses after delivery of the TTFC antigen expressed either on the spore surface or in the germinating spore were compared [87]. Antigen on the spore surface produced a more pronounced, but less rapid, response with a type 1 T cell response bias.

The protective antigen (PA) from a toxin-producing B. anthracis strain is a key protein for the interaction of a lethal factor and the host membrane, and neutralization of PA and prevention of its binding to the host cell surface was a main target for vaccine development. PA has been shown to induce protection against infection by that strain [88]. Besides oral and mucosal delivery, parenteral delivery of B. subtilis spores carrying PA was tested [89]. Spores carrying different (31, 48, and 91 kDa) domains of the PA at the C-terminal end of CotB or CotC were delivered into six mice through the intra-peritoneal route, and were very effective to confer protective immunity using an in vitro toxin neutralization assay and a challenge experiment with the latter showing protection to a lethal dose of B. anthracis spore.

Besides CotB, the CotC protein has also been used as an anchoring motif for the display of TTFC and the LTB (heat-labile toxin of E. coli) antigen [90]. In both cases, the antigen-displaying spores provoked an immune response in mice, when they were delivered by intra-peritoneal injection or by oral administration. A Chinese group published two related reports for the development of spore-based vaccines against human clonorchiasis using CotC as an anchoring motif [91, 92]. Caused by infection with the nematode of C. sinensis, clonorchiasis is endemic in southern China [93], Korea, and other southeast Asian countries. By large-scale sequencing of a C. sinensis cDNA library, they had identified three full-length cDNAs encoding three putative tegumental proteins (TP20.8, TP22.3, TP31.8), which could be the most susceptible target for vaccines because of their importance for the host response and parasite survival. Among them, they performed parallel study with TP20.8 [94] and TP22.3 [91]. In both cases, when antigen-displaying spores were delivered orally, they reported an elevated secretory level of IgA, which aggregated pathogens, inhibited their motility, and prevented their adherence to epithelial cells. However, the protection rate against metacercariae challenge had no significant differences compared with those of control groups, when spore-displaying TP20.8 was delivered. Only spore-expressing TP22.3 could induce a significant level of protection evidenced as reduced worm burden in the vaccinated rats (44.7%), suggesting that TP20.8 is a real membrane protein with a primary transmembrane helix as an interesting vaccine candidate.

In a recent publication [95], comparison of antigenicity and immunogenicity of a T helper epitope pep23 expressed in three different vaccine delivery systems was carried out. In an experiment performed by using a specific T cell hybridoma and by priming mononuclear cells isolated from the venous blood of human donors, spore display system with fusion at the N-terminal of CotC was less effective when compared to filamentous bacteriophage fd display system or cell surface display system based on E2 protein from the PDH complex of the B. stearothermophilus system. However, considering the safety and ease of mass production, Bacillus spores have many valuable applications in mucosal administration.

In a completely different application using CotG and CotE as anchoring motives, β-galactosidase of E. coli, the most utilized reporter protein in bioscience and biotechnology, was surface-expressed on B. subtilis spores [96]. β-Galactosidase is a very large protein (116 kDa per monomer), and active only as a tetramer and known to be toxic to the host cell when translocation was tried using the Sec pathway of host cells causing membrane jamming. β-Galactosidase was fused to the C-terminal ends of CotG and CotE each. Its surface expression was verified by flow cytometry and by a protease accessibility test. This result demonstrated the advantage of a spore-based display system compared to the vegetative cell-based approach in that it has obviously no limit in size (116 × 4 = 464 kDa) and in the multimeric nature of the target protein.

Besides hydrolyzing lactose to glucose and galactose, β-galactosidase can also catalyze a transglycosylation reaction in water-organic solvent biphasic reaction systems [97]. With the spore surface-anchored β-galactosidase, alkyl-β-galactosides, a group of nonionic surfactants, which exhibit antimicrobial activity and are used as drug intermediates, could be produced [98]. Solvent stability of surface expressed β-galactosidase, especially in ethyl ether, toluene, ethyl acetate, and acetonitrile was significantly increased as compared to the free form of soluble β-galactosidase. And the thermostability of surface-expressed β-galactosidase was also increased compared to free soluble β-galactosidase. Through this approach, the problem of low stability of the enzyme in solvents and a strong limitation in mass transfer between water and solvent phases separating enzymes and substrates could be eliminated.

In another application, streptavidin was immobilized on the spore surface [77]. Streptavidin is a 60-kDa protein produced by Streptomyces avidinii and binds biotin very tightly with a K _d of 10⁻¹⁵. Active streptavidin is composed of four identical molecules with a molecular weight of 15 kDa each, and four active sites for biotin binding exist in the active tetramer. Biotin can be easily conjugated to various biomolecules, such as proteins, nucleic acids, and carbohydrates, and spore-displayed streptavidin can be widely used for the detection of biotin-conjugated biomolecules. But the high G + C content of the DNA sequence (69%) and sequestration of intracellular biotin, which is lethal to the host bacteria, and tetramer formation of streptavidin makes it difficult to express streptavidin intracellularly or to secrete it in its active form. Using the CotG anchoring motif, they generated streptavidin-coated B. subtilis spores, and its localization was confirmed by anti-streptavidin antibodies. Furthermore, the biological activity of surface-expressed streptavidin was directly confirmed by Biotin-FITC conjugate by flow cytometry, and streptavidin-coated spores can directly recognize biotin-FITC on glass surfaces, confirmed by fluorescence microscopy. This application could provide a simple and superior platform compared to the B. thuringiensis micropatterns (see below) [99], eliminating antibody and biotin-protein A treatment on biotin-patterned surfaces.

In a third application, GFPuv, a variant of the green fluorescent protein, was surface-displayed on B. subtilis spores using the CotG anchoring motif [100]. Two different GFPuv display vectors (with and without the coding region of cotG) were constructed to examine the precise role of CotG as an anchoring motif for the display of GFPuv. As to be expected, only spores expressing the fusion protein exhibited fluorescence due to surface-anchored GFPuv. This application offers the interesting possibility to use GFPuv for the identification of additional spore coat proteins present on the outside such as CotU to be used as anchoring motif.

Anchoring of proteins on the spore surface of other endospore-formers

Many Bacillus species can be found in the soil, where the majority of these are saprophytes. But three species, B. cereus, B. anthracis, and B. thuringiensis, are pathogens of animals and insects. The spore architecture of these pathogens, as well as of some of the saprophytes, differs from that of B. subtilis in possessing an additional outermost layer called exosporium [101] (Fig. 2). The exosporium surrounds the spore coat and consists of a paracrystalline basal layer and an external hair-like nap. In B. anthracis, the basal layer contains more than a dozen different integral proteins, and the filaments of the hair-like nap are formed mainly by a single collagen-like protein called BclA [102] and the less frequent protein BclB [103]. Sequences near the N-terminus of both glycoproteins are responsible for incorporation of BclA and BclB into the exosporium layer of the spore. They were used to immobilize GFP onto the spore surface [104], suggesting a new vaccine delivery system based on inactivated spores of B. anthracis.

Spores of B. thuringiensis contain the 130-kDa protoxin from the Cry1Ac subgroup as a major component of the spore coat layer, which forms originally parasporal crystal inclusion outside the exosporium [105]. It has been proposed that the N-terminal part of the protoxin is exposed on the spore surface, while the C-terminal region anchors the protein inside the spore coat [106]. Two different passenger proteins have been used to replace the N-terminal end, and the chimeric proteins were successfully anchored on the spore surface: GFP and a single-chain antibody (scFv) recognizing a chemical compound [107]. The advantages of B. thuringiensis spores for surface display are the higher sporulation rate (98–100 vs 60–70% for B. subtilis) and the finding that the protoxin is not essential, while in B. subtilis both the native Cot proteins and the Cot fusion proteins are anchored on the surface of the spores.

Micropatterns are spatially well-defined, two-dimensional microstructures of, e.g., cells on a solid surface such as a glass slide, which can serve as sensors/detectors. Here, micropatterns of B. thuringiensis spores have been prepared [99]. First, EGFP was fused to the InhA protein, and these spores were then treated with anti-GFP antibodies, followed by addition of protein A-biotin. These complexes were added to a glass slide with immobilized streptavidin. The spores were analyzed under the microscope and exhibited an ellipsoidal shape. In addition, the authors could show that the immobilized spores were still able to germinate when the glass slides were placed with the upside-down configuration on LB agar plates [99]. All the major applications of proteins displayed on spore surface are listed in Table 1.

Table 1.

Summary of Bacillus spore surface-anchored proteins

Anchoring motif	Passenger	Host	Application	References
CotB	TTFC	B. subtilis	First report; spore display for vaccination	[84]
	TTFC	B. subtilis	Detailed analysis of immune response following oral/intranasal immunization of mice with spore	[85]
CotC	TTFC	B. subtilis	Vaccination by intra-peritoneal injection or oral administration	[90]
			LTB (heat labile toxin of E. coli)
	C. sinensis tegumental	B. subtilis	Surface display of parasite vaccine candidate TP20.8	[91]
	Protein TP20.8		Enhanced level of fecal secretory IgA
	C. sinensis tegumental	B. subtilis	Surface display of parasite vaccine candidate TP22.3	[92]
	Protein TP22.3		Significant protection rate against metacercariae challenge
CotB, CotC	Protective antigen (PA)	B. subtilis	Display of B. anthrax protective antigen (PA)	[89]
BclA	EGFP, DsRed	B. anthracis	Display on the exosporium	[104]
InhA	eGFP	B. thuringiensis	Micropatterns of EGPF spores	[99]
Cry1Ac	GFP, anti-phOx scFv	B. thuringiensis	Anchoring motif localization, anti-phOx scFv display	[107]
CotC	T helper epitope	B. stearothermophilus	Induction of a specific immune response	[99]
CotG	Streptavidin	B. subtilis	Display of toxic, tetrameric protein	[77]
	β-galactosidase	B. subtilis	Display of largest tetramer (4 × 116 kDa)	[98]
			Enzyme stability enhancement in organic bi-phasic reaction
	GFPuv	B. subtilis	Assessment of anchoring motif; signaling application	[100]

Concluding remarks

Spore display is an interesting alternative to phage and cell display. It offers four important advantages when compared to the other systems: (1) the fusion proteins do not have to cross a membrane; (2) ATP-dependent molecular chaperones are present and can assist correct folding of the fusion proteins; (3) spore display allows oligomerization of proteins as shown in the case of β-galactosidase; and (4) there is no obvious codon bias. Spores can be prepared at a high rate (~10¹⁰/ml) from flask cultures and can be stored for indefinite times after purification at room temperature. This allows the establishment of combinatorial libraries of, e.g., single chain libraries. One open question is why not all spores express the passenger protein on their surface. When spores are analyzed by flow cytometry, sometimes only about 50% of the spores exhibit a signal ([92], and our unpublished results). Using GFP as passenger protein, additional spore coat proteins can be identified being surface exposed such as CotU [79].