Figure 5: Application of IFDlong pipeline into single-cell long-read RNA-seq data on human HCC.

(A) t-SNE visualization on the single-cell data based on isoform expression profile. (B) Top KEGG pathways enriched by the differentially expressed isoforms (DEI) comparing tumor and normal cells. (C) Multiple alternative splicing events of gene CD74 present differential expression patterns between normal and tumor cells.