Figure 5. Synovial tumors harbor broad H3K4me3 domains.

(a) Unsupervised hierarchal clustering (distance = 1 - spearman correlation, ward.D2 clustering method) of H3K4me3 in primary SyS (pink) and other normal and diseased tissue from the International Human Epigenome Consortium (IHEC). Heat is the proportion of promoter regions marked by H3K4me3 peaks. Box plots showing (b) the genomic occupancy of H3K4me3, (c) mean peak width of H3K4me3 at promoter regions and (d) fractional methylation of promoters in SyS and other normal and diseased tissue from IHEC. (e) Mean methylation (beta values) of promoter regions in SyS and other sarcoma subtypes. (f) Gene Ontology analysis of genes whose promoters are overlap differentially hypomethylated regions (DMRs) in SyS compared to other sarcoma subtypes. * indicates P-value < 0.05 using pairwise Wilcoxon signed-rank test.