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[Preprint]. 2024 May 17:2024.05.17.594752. [Version 1] doi: 10.1101/2024.05.17.594752

Aging leads to sex-dependent effects on pair bonding and increased number of oxytocin-producing neurons in monogamous prairie voles

Michael A Kelberman, Kelly E Winther, Yana M Medvedeva, Zoe R Donaldson
PMCID: PMC11118570  PMID: 38798336

Abstract

Pair bonds powerfully modulate health, which becomes particularly important when facing the detrimental effects of aging. To examine the impact of aging on relationship formation and response to loss, we examined behavior in 6-, 12-, and 18-month male and female prairie voles, a monogamous species that forms mating-based pair bonds. We found that older males (18-months) bonded quicker than younger voles, while similarly aged female voles increased partner directed affiliative behaviors. Supporting sex differences in bonding behaviors, we found that males were more likely to sample both partner and novel voles while females were more likely to display partner preference during the initial 20 minutes of the test. Using partner separation to study loss, we observed an erosion of partner preference only in 12-month females, but an overall decrease in partner-directed affiliation in females across all groups, but not in males. Finally, we found that the number of oxytocin, but not vasopressin, cells in the paraventricular hypothalamus increased during aging. These results establish prairie voles as a novel model to study the effects of normal and abnormal aging on pair bonding.

Highlights

  • 18-month male voles demonstrate accelerated bond formation

  • 18-month female voles increase partner-directed huddling after 2 wks

  • Bonds erode faster in 12-month female voles after partner separation

  • Female behavior from partner preference tests is reflected in free interaction

  • The number of paraventricular hypothalamus oxytocin cells increase during aging

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