Figure 1.

Embedding mouse brains using forms constructed from microscope slides. (A) Rectangular forms were constructed from five microscope slides or optionally on a 3D printer. (B) The form was placed on a cold plate and the intersections of the slides were sealed with gelatin solution. (C) A 2 mm gelatin base was poured and allowed to harden. (D) Brains were transferred from the gelatin solution at 42 °C to the form and allowed to set for 5 min. (E) Gelatin was carefully poured over the brains to a level of 2–3 mm and allowed to set for 5 min. (F) The block was placed in the cold room for 1 h, removed from the form, and trimmed to remove excess gelatin.